{"id":"ba591ea6-1a54-5865-a5d9-9f92206a51b4","stable_key":"e3f6ce02-0bd7-5103-acc1-68503ab6751b:b5-coa-backbone","predicate":"reported_relationship","statement":"Labeled pantothenate supplied the CoA backbone used to trace mitochondrial CoASH import in human cells.","claim_class":"mechanistic","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"0b4d666a-9be2-5eb7-bd98-c49ec51adb9e","mechanism_event_label":"Labeled pantothenate supplied the CoA backbone used to trace mitochondrial CoASH import in human cells.","subject":{"id":"7bc62072-9517-5563-a3b8-8c5ab64a464b","slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"},"object":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"0b4d666a-9be2-5eb7-bd98-c49ec51adb9e","stable_key":"e3f6ce02-0bd7-5103-acc1-68503ab6751b:b5-coa-backbone-event","event_type":"biochemical_relationship","label":"Labeled pantothenate supplied the CoA backbone used to trace mitochondrial CoASH import in human cells.","description":"### b5-coa-backbone\n\nLabeled pantothenate supplied the CoA backbone used to trace mitochondrial CoASH import in human cells.\nThe isotope labels were on the CoA moiety, not its attached acetyl group. This connects vitamin B5 supply to the cofactor required for ALCAR use without asserting a benefit from excess B5.\nModel and exposure: Human K562 cells; 13C3-15N-pantothenate labeling for three days followed by acyl-CoA extraction and mitochondrial uptake assay; Fig. 7a.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Cellular pan-chain acyl-CoA profiling reveals SLC25A42/SLC25A16 in mitochondrial CoA import and metabolism. | 2025 | https://pubmed.ncbi.nlm.nih.gov/40925986/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC12460162/ | https://doi.org/10.1038/s42255-025-01358-y | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7bc62072-9517-5563-a3b8-8c5ab64a464b","slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"},"role":"recorded factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""},{"entity":{"id":"010f4333-18ae-50cb-8066-e42cf790b60e","slug":"human-slc25a16","display_name":"Human mitochondrial CoA transporter SLC25A16","entity_type_key":"protein"},"role":"delivery machinery","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"14124a14-1eb8-5e7b-89d2-ad6ed3409238","slug":"slc25a42","display_name":"Human mitochondrial coenzyme A transporter SLC25A42","entity_type_key":"protein"},"role":"delivery machinery","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human K562 cells; 13C3-15N-pantothenate labeling for three days followed by acyl-CoA extraction and mitochondrial uptake assay; Fig. 7a.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived curation with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Acetyl-L-carnitine collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Labeled pantothenate supplied the CoA backbone used to trace mitochondrial CoASH import in human cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cellular pan-chain acyl-CoA profiling reveals SLC25A42/SLC25A16 in mitochondrial CoA import and metabolism. | https://pubmed.ncbi.nlm.nih.gov/40925986/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC12460162/ | https://doi.org/10.1038/s42255-025-01358-y","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference b5-coa-backbone; The isotope labels were on the CoA moiety, not its attached acetyl group. This connects vitamin B5 supply to the cofactor required for ALCAR use without asserting a benefit from excess B5.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2c2c830f-3620-5206-b4a5-6794eeb1ab93","evidence_kind":"source_excerpt","locator":"Lines 52-58","start_line":52,"end_line":58,"excerpt":"### b5-coa-backbone\n\nLabeled pantothenate supplied the CoA backbone used to trace mitochondrial CoASH import in human cells.\nThe isotope labels were on the CoA moiety, not its attached acetyl group. This connects vitamin B5 supply to the cofactor required for ALCAR use without asserting a benefit from excess B5.\nModel and exposure: Human K562 cells; 13C3-15N-pantothenate labeling for three days followed by acyl-CoA extraction and mitochondrial uptake assay; Fig. 7a.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Cellular pan-chain acyl-CoA profiling reveals SLC25A42/SLC25A16 in mitochondrial CoA import and metabolism. | 2025 | https://pubmed.ncbi.nlm.nih.gov/40925986/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC12460162/ | https://doi.org/10.1038/s42255-025-01358-y | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"Human K562 cells; 13C3-15N-pantothenate labeling for three days followed by acyl-CoA extraction and mitochondrial uptake assay; Fig. 7a.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2588f904-245a-597a-8a12-65118013fb90","stable_key":"import-e3f6ce02-0bd7-5103-acc1-68503ab6751b","title":"Acetyl-L-carnitine: detailed mechanisms of action (reviewed 6 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original review of primary studies and clearly identified official records. Exact primary access is stated; no independent replication is claimed.","file_path":"","sha256":"84cc82d6fbb0fd5d73c4ce779e9f88e7f0184ba2d29b18ad0f153815380f2975","revision_id":"dac7a950-dbe5-5c7a-9c70-2ea711d5c71a","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}