{"id":"b9f24db0-aa60-5469-b65f-a1f50b3f3f7b","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-hydroxyl-assay","predicate":"decreases_in_recorded_comparison","statement":"RA reduced hydroxyl-radical signal in the 2016 ra assay, IC50 29.4 micromolar.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"2eaf72b1-b2f4-5992-89da-67dc6441f007","mechanism_event_label":"RA reduced hydroxyl-radical signal in the 2016 ra assay, IC50 29.4 micromolar.","subject":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"object":{"id":"6ae72aa4-6657-5681-a216-80c122ff720c","slug":"ra-hydroxyl-radical-assay","display_name":"Hydroxyl-radical signal in the 2016 RA assay","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2eaf72b1-b2f4-5992-89da-67dc6441f007","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-hydroxyl-assay-event","event_type":"experimental_observation","label":"RA reduced hydroxyl-radical signal in the 2016 ra assay, IC50 29.4 micromolar.","description":"### ra-hydroxyl-assay\n\nRA reduced hydroxyl-radical signal in the 2016 ra assay, IC50 29.4 micromolar.\nRadical-scavenging activity does not establish a selective protein target or net antioxidant effect in humans.\nModel and exposure: 2016 chemical assays; assay-specific endpoints.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Combining in vitro and in silico approaches to evaluate the multifunctional profile of rosmarinic acid from Blechnum brasiliense on targets related to neurodegeneration. | 2016 | https://pubmed.ncbi.nlm.nih.gov/27270453/ | https://doi.org/10.1016/j.cbi.2016.06.005 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"RA concentration series","sequence_order":0,"notes":""},{"entity":{"id":"6ae72aa4-6657-5681-a216-80c122ff720c","slug":"ra-hydroxyl-radical-assay","display_name":"Hydroxyl-radical signal in the 2016 RA assay","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"RA concentration series\", \"comparator\": \"Assay without RA\", \"endpoint\": \"RA reduced hydroxyl-radical signal in the 2016 ra assay, IC50 29.4 micromolar.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"2016 chemical assays; assay-specific endpoints.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Rosmarinic acid collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"RA reduced hydroxyl-radical signal in the 2016 ra assay, IC50 29.4 micromolar.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Combining in vitro and in silico approaches to evaluate the multifunctional profile of rosmarinic acid from Blechnum brasiliense on targets related to neurodegeneration. | https://pubmed.ncbi.nlm.nih.gov/27270453/ | https://doi.org/10.1016/j.cbi.2016.06.005","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference ra-hydroxyl-assay; Radical-scavenging activity does not establish a selective protein target or net antioxidant effect in humans.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"499d4420-d083-51d8-9fb8-b71c17290de2","evidence_kind":"source_excerpt","locator":"Lines 590-596","start_line":590,"end_line":596,"excerpt":"### ra-hydroxyl-assay\n\nRA reduced hydroxyl-radical signal in the 2016 ra assay, IC50 29.4 micromolar.\nRadical-scavenging activity does not establish a selective protein target or net antioxidant effect in humans.\nModel and exposure: 2016 chemical assays; assay-specific endpoints.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Combining in vitro and in silico approaches to evaluate the multifunctional profile of rosmarinic acid from Blechnum brasiliense on targets related to neurodegeneration. | 2016 | https://pubmed.ncbi.nlm.nih.gov/27270453/ | https://doi.org/10.1016/j.cbi.2016.06.005 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","model_system":"2016 chemical assays; assay-specific endpoints.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"606cff22-a6ed-59cd-a84d-87f44c59dc25","stable_key":"import-ca8e9e6a-6953-51ec-b712-2b06398a416d","title":"Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"684b4eab1dc2c0057f679109770af0de9340af5cd933377b590298cf8a752956","revision_id":"4028d9c5-fb17-54c5-b72c-c6d21678a832","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}