{"id":"b9e9acc4-14d5-5f80-ae91-2b462c720108","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-hdac-hdac2","predicate":"promotes_proteasomal_degradation_of","statement":"DIM promoted proteasomal depletion of HDAC2 in the colon-cancer study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ca177b73-c46e-5c1a-9ae0-f81fe5dd969f","mechanism_event_label":"This mechanism removes an epigenetic regulatory protein rather than merely blocking its active site.","subject":{"id":"6009bdd4-f1b5-5867-b5c3-cdf7c9645cd0","slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"},"object":{"id":"c1d729d9-230b-5dad-a09f-3e6b46a700ea","slug":"hdac2","display_name":"Human histone deacetylase 2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ca177b73-c46e-5c1a-9ae0-f81fe5dd969f","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-hdac-hdac2-event","event_type":"biochemical_relationship","label":"This mechanism removes an epigenetic regulatory protein rather than merely blocking its active site.","description":"DIM promoted proteasomal depletion of HDAC2 in the colon-cancer study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6009bdd4-f1b5-5867-b5c3-cdf7c9645cd0","slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c1d729d9-230b-5dad-a09f-3e6b46a700ea","slug":"hdac2","display_name":"Human histone deacetylase 2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/dim-research/20068155.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"bbb7efbd817b44da2771c9dac2c27d0bf4fe3db97ad9452a93eefc97319ebab9\", \"start_char\": 0, \"end_char\": 862, \"text_sha256\": \"bbb7efbd817b44da2771c9dac2c27d0bf4fe3db97ad9452a93eefc97319ebab9\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Protein degradation and cell-cycle studies","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"DIM; proteasome-mediated protein depletion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Preclinical concentration/model dependence. The mechanism is loss of HDAC protein, not established direct inhibition of every HDAC catalytic site.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Diindolylmethane (DIM) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human colon-cancer cells and xenografts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This mechanism removes an epigenetic regulatory protein rather than merely blocking its active site.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[dim-p20068155] Chemopreventive agent 3,3'-diindolylmethane selectively induces proteasomal degradation of class I histone deacetylases. (2010). https://pubmed.ncbi.nlm.nih.gov/20068155/ DOI: 10.1158/0008-5472.can-09-1924","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Class I HDAC regulation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a052502b-205c-5b67-b1f4-68eba28f9d9a","evidence_kind":"source_excerpt","locator":"Lines 1377-1388","start_line":1377,"end_line":1388,"excerpt":"### dim-hdac-hdac2\nDIM promoted proteasomal depletion of HDAC2 in the colon-cancer study.\nCondition category: normal\nnutrient_topic: Diindolylmethane (DIM) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This mechanism removes an epigenetic regulatory protein rather than merely blocking its active site.\norganism: Human colon-cancer cells and xenografts\ntissue_or_cell_type: Class I HDAC regulation\nexperimental_model: Protein degradation and cell-cycle studies\nlimitations: Preclinical concentration/model dependence. The mechanism is loss of HDAC protein, not established direct inhibition of every HDAC catalytic site.\nexposure: DIM; proteasome-mediated protein depletion\nevidence_span: {\"source_cache\": \"artifacts/dim-research/20068155.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"bbb7efbd817b44da2771c9dac2c27d0bf4fe3db97ad9452a93eefc97319ebab9\", \"start_char\": 0, \"end_char\": 862, \"text_sha256\": \"bbb7efbd817b44da2771c9dac2c27d0bf4fe3db97ad9452a93eefc97319ebab9\"}\n[dim-p20068155] Chemopreventive agent 3,3'-diindolylmethane selectively induces proteasomal degradation of class I histone deacetylases. (2010). https://pubmed.ncbi.nlm.nih.gov/20068155/ DOI: 10.1158/0008-5472.can-09-1924","model_system":"Protein degradation and cell-cycle studies","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [dim-p20068155] Chemopreventive agent 3,3'-diindolylmethane selectively induces proteasomal degradation of class I histone deacetylases. (2010). https://pubmed.ncbi.nlm.nih.gov/20068155/ DOI: 10.1158/0008-5472.can-09-1924","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b6d70682-97f9-5893-a03c-f9f88836033c","stable_key":"import-182336c6-ed36-5ec6-8a09-25c31096262e","title":"Diindolylmethane (DIM): formation, receptor signaling, metabolism and drug interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"31e14060de904976d2f5d439f2bc9c8a99a72d34626c562c78429025e050d404","revision_id":"611094c1-a9cf-5b9b-b255-ac70b571696a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}