{"id":"b9bc943b-df64-5ac2-8333-3efb9fe02aaa","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-catalase","predicate":"attenuates","statement":"Added catalase substantially reduced EGCG cytotoxicity in the culture comparison.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"6c207d60-f41f-5c24-abba-debc66a8ac91","mechanism_event_label":"Removing peroxide reduced the observed cell injury.","subject":{"id":"6a0fc815-8990-50bf-b164-16e14138ffc6","slug":"experimental-catalase","display_name":"Experimental catalase preparation","entity_type_key":"protein_family"},"object":{"id":"c65d88e5-0f47-52f9-99fa-e8b7b7a76bdc","slug":"egcg-cho-cytotoxicity","display_name":"EGCG cytotoxicity in CHO cell cultures","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6c207d60-f41f-5c24-abba-debc66a8ac91","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-catalase-event","event_type":"observed_relationship","label":"Removing peroxide reduced the observed cell injury.","description":"Added catalase substantially reduced EGCG cytotoxicity in the culture comparison.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6a0fc815-8990-50bf-b164-16e14138ffc6","slug":"experimental-catalase","display_name":"Experimental catalase preparation","entity_type_key":"protein_family"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c65d88e5-0f47-52f9-99fa-e8b7b7a76bdc","slug":"egcg-cho-cytotoxicity","display_name":"EGCG cytotoxicity in CHO cell cultures","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"22e1b8eb-f35f-5afa-be20-b0534df9f6be","slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"CHO-cell experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Rescue implicates peroxide in this setting; it does not establish oral catalase or antioxidant combinations as treatment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"EGCG collection; comparator and shared-pathway records retain their actual intervention.","comparator":null,"unit":null,"notes":"","entity":{"slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Removing peroxide reduced the observed cell injury.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Different cytotoxic and clastogenic effects of epigallocatechin gallate in various cell-culture media due to variable rates of its oxidation in the culture medium. · 2007 · https://pubmed.ncbi.nlm.nih.gov/17851114/ · DOI 10.1016/j.mrgentox.2007.07.009","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cb398a3a-e83e-5549-a156-48ba4e123242","evidence_kind":"source_excerpt","locator":"Lines 340-346","start_line":340,"end_line":346,"excerpt":"## egcg-catalase\nRemoving peroxide reduced the observed cell injury.\nAdded catalase substantially reduced EGCG cytotoxicity in the culture comparison.\nModel: CHO-cell experiments.\nLimitations: Rescue implicates peroxide in this setting; it does not establish oral catalase or antioxidant combinations as treatment.\nEvidence access: primary abstract.\nDifferent cytotoxic and clastogenic effects of epigallocatechin gallate in various cell-culture media due to variable rates of its oxidation in the culture medium. · 2007 · https://pubmed.ncbi.nlm.nih.gov/17851114/ · DOI 10.1016/j.mrgentox.2007.07.009","model_system":"CHO-cell experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Primary-abstract paraphrase; no full-text methods verification claimed.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1f6d09e8-3aae-5c41-9ea7-15d0d1cab72d","stable_key":"import-ec987f3e-0b3c-55cc-b304-c703a738e35d","title":"EGCG: receptor signaling, metabolism, nutrient interactions and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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