{"id":"b9b6ae21-d545-563a-988f-84475244a837","stable_key":"8c946991-97f0-55ee-8df0-00e67621962f:nad-plus-slc52-expression","predicate":"overexpression_increases","statement":"Overexpression of human SLC25A52 increased mitochondrial NAD+ in cultured human cells and complemented yeast lacking endogenous mitochondrial NAD transporters.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b37992dc-a7f6-5ad6-b02d-c5fdd1bc9cc1","mechanism_event_label":"A related transporter can support mitochondrial NAD availability in experimental systems.","subject":{"id":"a848f6dd-cd4f-5508-96cc-9dcdf3a4da1e","slug":"slc25a52","display_name":"Human SLC25A52","entity_type_key":"protein"},"object":{"id":"bc083b46-b5c5-50f6-a084-28fdb2a96f49","slug":"mitochondrial-nad-plus-content","display_name":"Mitochondrial NAD+ content","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b37992dc-a7f6-5ad6-b02d-c5fdd1bc9cc1","stable_key":"8c946991-97f0-55ee-8df0-00e67621962f:nad-plus-slc52-expression-event","event_type":"observed_relationship","label":"A related transporter can support mitochondrial NAD availability in experimental systems.","description":"Overexpression of human SLC25A52 increased mitochondrial NAD+ in cultured human cells and complemented yeast lacking endogenous mitochondrial NAD transporters.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a848f6dd-cd4f-5508-96cc-9dcdf3a4da1e","slug":"slc25a52","display_name":"Human SLC25A52","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bc083b46-b5c5-50f6-a084-28fdb2a96f49","slug":"mitochondrial-nad-plus-content","display_name":"Mitochondrial NAD+ content","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human-cell overexpression and yeast complementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Overexpression does not establish normal tissue contribution, redundancy or human treatment efficacy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"NAD+ collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A related transporter can support mitochondrial NAD availability in experimental systems.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"SLC25A51 is a mammalian mitochondrial NAD+ transporter. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32906142/ · DOI 10.1038/s41586-020-2741-7","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"585d8bfc-6f58-553c-adda-7149ad8daf7a","evidence_kind":"source_excerpt","locator":"Lines 44-50","start_line":44,"end_line":50,"excerpt":"## nad-plus-slc52-expression\nA related transporter can support mitochondrial NAD availability in experimental systems.\nOverexpression of human SLC25A52 increased mitochondrial NAD+ in cultured human cells and complemented yeast lacking endogenous mitochondrial NAD transporters.\nModel: Human-cell overexpression and yeast complementation.\nLimitations: Overexpression does not establish normal tissue contribution, redundancy or human treatment efficacy.\nEvidence access: Primary full text\nSLC25A51 is a mammalian mitochondrial NAD+ transporter. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32906142/ · DOI 10.1038/s41586-020-2741-7","model_system":"Human-cell overexpression and yeast complementation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f59aa302-d463-5462-876f-e21e64861eac","stable_key":"import-8c946991-97f0-55ee-8df0-00e67621962f","title":"NAD+: compartmental supply, consumption and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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