{"id":"b97f4191-116d-5cbc-88ab-990fdd038ad0","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-gsh-rescue","predicate":"prevents","statement":"Glutathione ethyl ester raised intracellular glutathione and completely protected cultured rat liver cells from eugenol- or quinone-methide-induced death.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"a08be10f-d735-58e3-8423-13233d0fe820","mechanism_event_label":"Glutathione ethyl ester raised intracellular glutathione and completely protected cultured rat liver cells from eugenol- or quinone-methide-induced death.","subject":{"id":"141b4c1f-7ac7-5ac6-ace4-a3233f9ce405","slug":"glutathione-ethyl-ester","display_name":"Glutathione ethyl ester","entity_type_key":"small_molecule"},"object":{"id":"441d7446-7707-58f2-ba46-adbf105593c8","slug":"eugenol-quinone-methide-cell-death","display_name":"Cell death caused by eugenol or its quinone-methide metabolite","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a08be10f-d735-58e3-8423-13233d0fe820","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-gsh-rescue-event","event_type":"observed_relationship","label":"Glutathione ethyl ester raised intracellular glutathione and completely protected cultured rat liver cells from eugenol- or quinone-methide-induced death.","description":"Glutathione ethyl ester raised intracellular glutathione and completely protected cultured rat liver cells from eugenol- or quinone-methide-induced death.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"141b4c1f-7ac7-5ac6-ace4-a3233f9ce405","slug":"glutathione-ethyl-ester","display_name":"Glutathione ethyl ester","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"441d7446-7707-58f2-ba46-adbf105593c8","slug":"eugenol-quinone-methide-cell-death","display_name":"Cell death caused by eugenol or its quinone-methide metabolite","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"d67cd5c6-7764-5cd5-a43b-5b368f2cb441","slug":"eugenol-quinone-methide","display_name":"Eugenol quinone-methide intermediate","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"dose","value_text":"Eugenol or quinone methide with glutathione ethyl ester","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Minutes to cell-death assessment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cultured rat liver Clone 9 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A cell-permeable experimental glutathione ester is not equivalent to dietary glutathione supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Eugenol chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Cultured rat liver Clone 9 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Glutathione ethyl ester raised intracellular glutathione and completely protected cultured rat liver cells from eugenol- or quinone-methide-induced death.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Comparative toxicity of eugenol and its quinone methide metabolite in cultured liver cells using kinetic fluorescence bioassays. 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(1998). https://pubmed.ncbi.nlm.nih.gov/9512727/ DOI: 10.1006/taap.1997.8348\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Cultured rat liver Clone 9 cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f2ff5a4-7264-57fc-8368-46b9768ec668","stable_key":"import-2f309d3a-d252-5dfb-ae24-993a625f1b6c","title":"Eugenol: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. 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