{"id":"b966a336-2ad2-56c4-af13-af33b030f402","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-magnesium-nmda","predicate":"shortens_agonist_channel_opening","statement":"Adding 30 micromolar magnesium reduced quinolinate-activated NMDA channel mean open time to about one-third at minus 100 mV.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6a51fd39-5438-5f3a-ab9c-cb05a61ca364","mechanism_event_label":"Magnesium and membrane voltage changed the receptor response.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"5050009a-583a-5618-bc59-ef31f4014224","slug":"rat-nmda-receptors","display_name":"Rat NMDA-type glutamate receptors, subunits unresolved","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"6a51fd39-5438-5f3a-ab9c-cb05a61ca364","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-magnesium-nmda-event","event_type":"observed_relationship","label":"Magnesium and membrane voltage changed the receptor response.","description":"Adding 30 micromolar magnesium reduced quinolinate-activated NMDA channel mean open time to about one-third at minus 100 mV.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5050009a-583a-5618-bc59-ef31f4014224","slug":"rat-nmda-receptors","display_name":"Rat NMDA-type glutamate receptors, subunits unresolved","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ab444b3c-c8ec-5ada-8450-36ac9934d6b6","slug":"quinolinic-acid","display_name":"Quinolinic acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat hippocampal patch-clamp experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not establish that oral magnesium prevents quinolinate-related disease.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Magnesium and membrane voltage changed the receptor response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Quinolinate activation of N-methyl-D-aspartate ion channels in rat hippocampal neurons. · 1990 · https://pubmed.ncbi.nlm.nih.gov/1700844/ · DOI 10.1016/0304-3940(90)90098-t","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b1aade22-daf7-590f-85d4-077014879f96","evidence_kind":"source_excerpt","locator":"Lines 282-288","start_line":282,"end_line":288,"excerpt":"## tryptophan-magnesium-nmda\nMagnesium and membrane voltage changed the receptor response.\nAdding 30 micromolar magnesium reduced quinolinate-activated NMDA channel mean open time to about one-third at minus 100 mV.\nModel: Rat hippocampal patch-clamp experiment.\nLimitations: Does not establish that oral magnesium prevents quinolinate-related disease.\nEvidence access: Primary abstract\nQuinolinate activation of N-methyl-D-aspartate ion channels in rat hippocampal neurons. · 1990 · https://pubmed.ncbi.nlm.nih.gov/1700844/ · DOI 10.1016/0304-3940(90)90098-t","model_system":"Rat hippocampal patch-clamp experiment.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7dfd20063b91900cb5c6ad675e3e675b7e25ef0fb8304eabac6f263cc5518284","revision_id":"bfafd789-fdf5-521f-bd28-c662e01ec1ea","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}