{"id":"b9413096-2997-519e-b3a7-b26d5e87caa8","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-islet-pyruvate-kinase","predicate":"inhibits","statement":"Alanine inhibited pyruvate kinase activity in normal rat islet homogenates.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"8b53a530-72e5-5e40-87ca-17410cf21680","mechanism_event_label":"A substrate entering one pathway can also regulate another metabolic step.","subject":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"object":{"id":"5bb814bd-070b-5455-87e4-107b11a9a543","slug":"rat-islet-pyruvate-kinase-assay","display_name":"Pyruvate kinase activity in normal rat islet homogenates","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"8b53a530-72e5-5e40-87ca-17410cf21680","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-islet-pyruvate-kinase-event","event_type":"observed_relationship","label":"A substrate entering one pathway can also regulate another metabolic step.","description":"Alanine inhibited pyruvate kinase activity in normal rat islet homogenates.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5bb814bd-070b-5455-87e4-107b11a9a543","slug":"rat-islet-pyruvate-kinase-assay","display_name":"Pyruvate kinase activity in normal rat islet homogenates","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"91e6d4f5-fba1-542d-b68a-57cc28e1e425","slug":"pyruvate","display_name":"Pyruvate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat islet homogenate enzyme assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Isoform not resolved from the accessed abstract; do not automatically assign PKM2 or a human protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A substrate entering one pathway can also regulate another metabolic step.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The stimulus-secretion coupling of amino acid-induced insulin release. Insulinotropic action of L-alanine. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12383948/ · DOI 10.1016/s0304-4165(02)00337-9","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e8829ba9-ff93-500c-8666-8cf8b9b05143","evidence_kind":"source_excerpt","locator":"Lines 392-398","start_line":392,"end_line":398,"excerpt":"## alanine-islet-pyruvate-kinase\nA substrate entering one pathway can also regulate another metabolic step.\nAlanine inhibited pyruvate kinase activity in normal rat islet homogenates.\nModel: Rat islet homogenate enzyme assay.\nLimitations: Isoform not resolved from the accessed abstract; do not automatically assign PKM2 or a human protein.\nEvidence access: Primary abstract\nThe stimulus-secretion coupling of amino acid-induced insulin release. Insulinotropic action of L-alanine. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12383948/ · DOI 10.1016/s0304-4165(02)00337-9","model_system":"Rat islet homogenate enzyme assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8ce7dd76c88b2e25fa23d93f9655a244aadb9d90f55ec8b18ecf0fd4625a61d2","revision_id":"4ed32258-0213-599a-a272-6f8f62280773","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}