{"id":"b9329cdf-ecd3-5580-94e4-ac860bf263dc","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-steviol-immune-null","predicate":"did_not_reproduce","statement":"Steviol at 100 micromolar did not reproduce the LPS-response suppression observed with 1 mM stevioside.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"0c3854b0-b329-58e8-9729-8ac08a38cf6a","mechanism_event_label":"Metabolism changes which immune effects are observed.","subject":{"id":"8ba32fe6-183f-5cd5-b386-02e6440e78a0","slug":"steviol","display_name":"Steviol","entity_type_key":"small_molecule"},"object":{"id":"3d8d3010-7667-5664-b73d-e9f7199d6c10","slug":"human-thp1-lps-cytokine-release","display_name":"Human THP-1 LPS-induced cytokine release","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0c3854b0-b329-58e8-9729-8ac08a38cf6a","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-steviol-immune-null-event","event_type":"observed_relationship","label":"Metabolism changes which immune effects are observed.","description":"Steviol at 100 micromolar did not reproduce the LPS-response suppression observed with 1 mM stevioside.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8ba32fe6-183f-5cd5-b386-02e6440e78a0","slug":"steviol","display_name":"Steviol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3d8d3010-7667-5664-b73d-e9f7199d6c10","slug":"human-thp1-lps-cytokine-release","display_name":"Human THP-1 LPS-induced cytokine release","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c78086c3-7657-589b-b353-1691df2d9f8f","slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"06e03b41-c225-5fa3-8aa3-13a07a9826a9","slug":"stevioside","display_name":"Stevioside","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human THP-1 assay comparing distinct compounds at different concentrations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Different doses prevent a simple potency ranking; no universal inactivity claim is made.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Metabolism changes which immune effects are observed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Anti-Inflammatory and Immunomodulatory Activities of Stevioside and Its Metabolite Steviol on THP-1 Cells. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16448183/ · DOI 10.1021/jf0523465","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c736df97-143f-50e8-a2e5-746d90475e48","evidence_kind":"source_excerpt","locator":"Lines 338-344","start_line":338,"end_line":344,"excerpt":"## stevia-steviol-immune-null\nMetabolism changes which immune effects are observed.\nSteviol at 100 micromolar did not reproduce the LPS-response suppression observed with 1 mM stevioside.\nModel: Human THP-1 assay comparing distinct compounds at different concentrations.\nLimitations: Different doses prevent a simple potency ranking; no universal inactivity claim is made.\nEvidence access: Primary abstract\nAnti-Inflammatory and Immunomodulatory Activities of Stevioside and Its Metabolite Steviol on THP-1 Cells. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16448183/ · DOI 10.1021/jf0523465","model_system":"Human THP-1 assay comparing distinct compounds at different concentrations.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"295304c9-609c-5b05-a18f-a00ab7b965ed","stable_key":"import-6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7","title":"Stevia: glycoside metabolism, taste, ion channels and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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