{"id":"b8f4ae51-603b-55c6-b777-ca79ab77da53","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-pcc-pcca","predicate":"provides_biotin_carboxylation_and_carrier_domains","statement":"PCCA contains the biotin-carboxylase and biotin-carrier domains of human PCC.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ccee2960-3d10-5168-a29e-473fb9797bd6","mechanism_event_label":"The alpha subunit handles biotin loading with the carboxyl group and carries that group onward.","subject":{"id":"9cd8356f-9496-51f7-9d0b-270019e39810","slug":"pcca","display_name":"Human propionyl-CoA carboxylase alpha subunit / PCCA","entity_type_key":"protein"},"object":{"id":"54eb0f2f-091c-54cd-b4a1-a528b994560a","slug":"human-propionyl-coa-carboxylase","display_name":"Human propionyl-CoA carboxylase / PCC","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"ccee2960-3d10-5168-a29e-473fb9797bd6","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-pcc-pcca-event","event_type":"biochemical_relationship","label":"The alpha subunit handles biotin loading with the carboxyl group and carries that group onward.","description":"PCCA contains the biotin-carboxylase and biotin-carrier domains of human PCC.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"tethered carrier","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"carboxylation energy substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9cd8356f-9496-51f7-9d0b-270019e39810","slug":"pcca","display_name":"Human propionyl-CoA carboxylase alpha subunit / PCCA","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"54eb0f2f-091c-54cd-b4a1-a528b994560a","slug":"human-propionyl-coa-carboxylase","display_name":"Human propionyl-CoA carboxylase / PCC","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/biotin-research/20725044.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"825bf28b9f35a8d45f8d08d883fa200bd430336b0558db035ce4fd1c5ef19053\", \"start_char\": 0, \"end_char\": 1921, \"text_sha256\": \"825bf28b9f35a8d45f8d08d883fa200bd430336b0558db035ce4fd1c5ef19053\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Bacterial PCC crystallography and separate 15-angstrom human PCC cryo-EM reconstruction","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Structural analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Atomic bacterial positions are not high-resolution human measurements. Human cryo-EM establishes the overall assembly at lower resolution.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Biotin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens; bacterial PCC comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The alpha subunit handles biotin loading with the carboxyl group and carries that group onward.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b7-p20725044] Crystal structure of the alpha(6)beta(6) holoenzyme of propionyl-coenzyme A carboxylase. (2010). https://pubmed.ncbi.nlm.nih.gov/20725044/ DOI: 10.1038/nature09302","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified PCC complexes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"071be063-beef-5d08-a799-4e06a51944fe","evidence_kind":"source_excerpt","locator":"Lines 598-609","start_line":598,"end_line":609,"excerpt":"### b7-pcc-pcca\nPCCA contains the biotin-carboxylase and biotin-carrier domains of human PCC.\nCondition category: normal\nnutrient_topic: Biotin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The alpha subunit handles biotin loading with the carboxyl group and carries that group onward.\norganism: Homo sapiens; bacterial PCC comparison\ntissue_or_cell_type: Purified PCC complexes\nexperimental_model: Bacterial PCC crystallography and separate 15-angstrom human PCC cryo-EM reconstruction\nlimitations: Atomic bacterial positions are not high-resolution human measurements. Human cryo-EM establishes the overall assembly at lower resolution.\nexposure: Structural analysis\nevidence_span: {\"source_cache\": \"artifacts/biotin-research/20725044.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"825bf28b9f35a8d45f8d08d883fa200bd430336b0558db035ce4fd1c5ef19053\", \"start_char\": 0, \"end_char\": 1921, \"text_sha256\": \"825bf28b9f35a8d45f8d08d883fa200bd430336b0558db035ce4fd1c5ef19053\"}\n[b7-p20725044] Crystal structure of the alpha(6)beta(6) holoenzyme of propionyl-coenzyme A carboxylase. (2010). https://pubmed.ncbi.nlm.nih.gov/20725044/ DOI: 10.1038/nature09302","model_system":"Bacterial PCC crystallography and separate 15-angstrom human PCC cryo-EM reconstruction","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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