{"id":"b86261c3-b167-5065-9980-42ed7740d238","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:b1-pdh-dlat-coa-acetylation","predicate":"transfers-acetyl-group-to","statement":"The human DLAT catalytic core contains a channel for the acetylated lipoyl group and CoA; substrate modeling positions CoA for acetyl-CoA formation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"9d0509c0-ea97-5701-aebc-85b4f2d3de64","mechanism_event_label":"After B1 acts at E1, the E2 protein transfers the acetyl group onto CoA, a vitamin B5-derived carrier.","subject":{"id":"12e22bc4-4bc8-5a45-87bc-9274656e9dab","slug":"dlat","display_name":"Dihydrolipoyl acetyltransferase / DLAT","entity_type_key":"protein"},"object":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"9d0509c0-ea97-5701-aebc-85b4f2d3de64","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:b1-pdh-dlat-coa-acetylation-event","event_type":"biochemical_relationship","label":"After B1 acts at E1, the E2 protein transfers the acetyl group onto CoA, a vitamin B5-derived carrier.","description":"The human DLAT catalytic core contains a channel for the acetylated lipoyl group and CoA; 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full source document retained when openly retrievable.\"}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human DLAT cryo-EM; bacterial ligand poses used for modeling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ligand positions were modeled, not directly resolved in a human substrate-bound structure; no intake experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient","value_text":"Thiamine (vitamin B1)","comparator":null,"unit":null,"notes":"","entity":{"slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"}},{"dimension":"nutrient_topic","value_text":"Thiamine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"After B1 acts at E1, the E2 protein transfers the acetyl group onto CoA, a vitamin B5-derived carrier.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[jiang-2018-pdh-core] Atomic Structure of the E2 Inner Core of Human Pyruvate Dehydrogenase Complex (2018). https://pubmed.ncbi.nlm.nih.gov/29608861/ DOI: 10.1021/acs.biochem.8b00357","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified catalytic core","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"52618c6f-55e5-5286-92f1-28d82a38c177","evidence_kind":"source_excerpt","locator":"Lines 690-702","start_line":690,"end_line":702,"excerpt":"### b1-pdh-dlat-coa-acetylation\nThe human DLAT catalytic core contains a channel for the acetylated lipoyl group and CoA; substrate modeling positions CoA for acetyl-CoA formation.\nCondition category: normal\nnutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: After B1 acts at E1, the E2 protein transfers the acetyl group onto CoA, a vitamin B5-derived carrier.\norganism: Homo sapiens\ntissue_or_cell_type: Purified catalytic core\nexperimental_model: Human DLAT cryo-EM; bacterial ligand poses used for modeling.\nlimitations: Ligand positions were modeled, not directly resolved in a human substrate-bound structure; no intake experiment.\nevidence: [{\"paper_key\": \"jiang-2018-pdh-core\", \"source_bundle\": \"artifacts/thiamine_metabolism_sources.json\", \"passage_ids\": [\"p-32\"], \"locator\": \"acetyl-accepting CoA\", \"preservation\": \"Exact text retained in the source bundle; full source document retained when openly retrievable.\"}]\ncross_nutrient: B1-dependent E1 and B5-derived CoA participate in different sequential steps.\nnutrient: Thiamine (vitamin B1)\n[jiang-2018-pdh-core] Atomic Structure of the E2 Inner Core of Human Pyruvate Dehydrogenase Complex (2018). https://pubmed.ncbi.nlm.nih.gov/29608861/ DOI: 10.1021/acs.biochem.8b00357","model_system":"Human DLAT cryo-EM; bacterial ligand poses used for modeling.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [jiang-2018-pdh-core] Atomic Structure of the E2 Inner Core of Human Pyruvate Dehydrogenase Complex (2018). https://pubmed.ncbi.nlm.nih.gov/29608861/ DOI: 10.1021/acs.biochem.8b00357","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"158d2c03-ac8c-589f-8270-c468165ae346","stable_key":"import-46d15d9e-d3b5-544d-ba01-b785aa3e4f42","title":"Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f376512fb3310141315548015b387833e3af45146e73fb73cd02cee20e4ddb9c","revision_id":"53bc5eda-dec8-58cc-a56f-a9eb4ab036ef","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}