{"id":"b82772ae-00c5-532a-8866-b116c3bcac1e","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-cutc-tma","predicate":"converts_choline_to","statement":"The characterized anaerobic choline-utilization pathway uses a glycyl-radical choline TMA-lyase to cleave the choline C–N bond and generate TMA.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"9a92b6cf-89df-5b22-bea4-24bb9c055bb1","mechanism_event_label":"Gut microbial machinery can divert choline into a different metabolic route.","subject":{"id":"49bc1c05-d37e-5382-821e-56373dd40a35","slug":"ds-cutc","display_name":"Desulfovibrio desulfuricans CutC choline TMA-lyase","entity_type_key":"protein"},"object":{"id":"ffdcd3fa-0e47-5c2b-96e2-00b7d1d5674e","slug":"trimethylamine","display_name":"Trimethylamine / TMA","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"9a92b6cf-89df-5b22-bea4-24bb9c055bb1","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-cutc-tma-event","event_type":"biochemical_relationship","label":"Gut microbial machinery can divert choline into a different metabolic route.","description":"The characterized anaerobic choline-utilization pathway uses a glycyl-radical choline TMA-lyase to cleave the choline C–N bond and generate TMA.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8e1d19fd-ad66-575d-81c4-9122f78915e5","slug":"choline","display_name":"Choline","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"49bc1c05-d37e-5382-821e-56373dd40a35","slug":"ds-cutc","display_name":"Desulfovibrio desulfuricans CutC choline TMA-lyase","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ffdcd3fa-0e47-5c2b-96e2-00b7d1d5674e","slug":"trimethylamine","display_name":"Trimethylamine / TMA","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/choline-research/23151509.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"20246eb796db8ca7b3ff78f530e744bd8f3abe8beb617358b0a1a924166fb2d6\", \"start_char\": 0, \"end_char\": 1160, \"text_sha256\": \"20246eb796db8ca7b3ff78f530e744bd8f3abe8beb617358b0a1a924166fb2d6\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Anaerobic bacterial genetics, heterologous expression and EPR","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Genetic knockout and heterologous expression of choline-utilization genes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A bacterial enzyme pathway, not a direct human-enzyme reaction or evidence that every microbiome produces the same TMA amount.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Choline research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"choline","display_name":"Choline","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Desulfovibrio desulfuricans; E. coli expression host","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Gut microbial machinery can divert choline into a different metabolic route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[choline-p23151509] Microbial conversion of choline to trimethylamine requires a glycyl radical enzyme. (2012). https://pubmed.ncbi.nlm.nih.gov/23151509/ DOI: 10.1073/pnas.1215689109","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Microbial choline-utilization pathway","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"79ffc748-f94a-579c-afef-9d1d20cc9849","evidence_kind":"source_excerpt","locator":"Lines 1101-1112","start_line":1101,"end_line":1112,"excerpt":"### choline-cutc-tma\nThe characterized anaerobic choline-utilization pathway uses a glycyl-radical choline TMA-lyase to cleave the choline C–N bond and generate TMA.\nCondition category: normal\nnutrient_topic: Choline research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Gut microbial machinery can divert choline into a different metabolic route.\norganism: Desulfovibrio desulfuricans; E. coli expression host\ntissue_or_cell_type: Microbial choline-utilization pathway\nexperimental_model: Anaerobic bacterial genetics, heterologous expression and EPR\nlimitations: A bacterial enzyme pathway, not a direct human-enzyme reaction or evidence that every microbiome produces the same TMA amount.\nexposure: Genetic knockout and heterologous expression of choline-utilization genes\nevidence_span: {\"source_cache\": \"artifacts/choline-research/23151509.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"20246eb796db8ca7b3ff78f530e744bd8f3abe8beb617358b0a1a924166fb2d6\", \"start_char\": 0, \"end_char\": 1160, \"text_sha256\": \"20246eb796db8ca7b3ff78f530e744bd8f3abe8beb617358b0a1a924166fb2d6\"}\n[choline-p23151509] Microbial conversion of choline to trimethylamine requires a glycyl radical enzyme. (2012). https://pubmed.ncbi.nlm.nih.gov/23151509/ DOI: 10.1073/pnas.1215689109","model_system":"Anaerobic bacterial genetics, heterologous expression and EPR","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [choline-p23151509] Microbial conversion of choline to trimethylamine requires a glycyl radical enzyme. (2012). https://pubmed.ncbi.nlm.nih.gov/23151509/ DOI: 10.1073/pnas.1215689109","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0ba2f05e-5cf5-5bd7-ae5d-0ab4945fcb7b","stable_key":"import-27e0c1cf-7726-5164-8b15-27a631584cd0","title":"Choline: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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