{"id":"b819b17c-391d-56b6-bde1-55c99da73bf8","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-fungal-atp","predicate":"increases_leakage","statement":"CHD-FA exposure caused extracellular ATP leakage from Candida cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"00b4a895-5c3f-523a-ae13-8455a330e96e","mechanism_event_label":"Cell contents escaped after membrane injury.","subject":{"id":"5184fd06-5a8e-5e9d-99ec-c2feaa59ccde","slug":"carbohydrate-derived-fulvic-acid","display_name":"Carbohydrate-derived fulvic acid / CHD-FA (preparation class)","entity_type_key":"chemical_species"},"object":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"00b4a895-5c3f-523a-ae13-8455a330e96e","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-fungal-atp-event","event_type":"observed_relationship","label":"Cell contents escaped after membrane injury.","description":"CHD-FA exposure caused extracellular ATP leakage from Candida cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5184fd06-5a8e-5e9d-99ec-c2feaa59ccde","slug":"carbohydrate-derived-fulvic-acid","display_name":"Carbohydrate-derived fulvic acid / CHD-FA (preparation class)","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0aa6c0fc-8374-56e0-b172-6c40b6366d21","slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Candida membrane-permeability assay; ATP release followed over time.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"ATP leakage indicates microbial membrane damage, not increased host ATP production.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fulvic acid collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Cell contents escaped after membrane injury.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Carbohydrate Derived Fulvic Acid: An in vitro Investigation of a Novel Membrane Active Antiseptic Agent Against Candida albicans Biofilms. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22479260/ · DOI 10.3389/fmicb.2012.00116","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e66b615c-4df7-5226-93a5-b24dc5ae9046","evidence_kind":"source_excerpt","locator":"Lines 132-138","start_line":132,"end_line":138,"excerpt":"## fulvic-acid-fungal-atp\nCell contents escaped after membrane injury.\nCHD-FA exposure caused extracellular ATP leakage from Candida cells.\nModel: Candida membrane-permeability assay; ATP release followed over time.\nLimitations: ATP leakage indicates microbial membrane damage, not increased host ATP production.\nEvidence access: Primary full text\nCarbohydrate Derived Fulvic Acid: An in vitro Investigation of a Novel Membrane Active Antiseptic Agent Against Candida albicans Biofilms. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22479260/ · DOI 10.3389/fmicb.2012.00116","model_system":"Candida membrane-permeability assay; ATP release followed over time.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b00a1818-46e4-52cc-8dfe-a647d5c08ed7","stable_key":"import-788beb7b-7928-5f89-83d4-5556abf3702c","title":"Fulvic acid: mixture identity, mineral chemistry, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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