{"id":"b8069cdb-d919-545b-9f3c-d65112d76787","stable_key":"f812c44f-d0fd-5f01-ba2c-748c0794fcfb:coumarin-1a2-epoxide","predicate":"catalyzes_formation_of","statement":"Recombinant human CYP1A2 catalyzed coumarin 3,4-epoxide formation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8aba3018-4015-51e3-a347-45c930654baf","mechanism_event_label":"CYP1A2 provides another route to the reactive intermediate.","subject":{"id":"9b15c13c-a369-5d10-a76a-525e8b19221d","slug":"cyp1a2","display_name":"Human cytochrome P450 1A2","entity_type_key":"protein"},"object":{"id":"07724327-ab75-5ac3-94ae-00cb0577b4c5","slug":"coumarin-3-4-epoxide","display_name":"Coumarin 3,4-epoxide","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"8aba3018-4015-51e3-a347-45c930654baf","stable_key":"f812c44f-d0fd-5f01-ba2c-748c0794fcfb:coumarin-1a2-epoxide-event","event_type":"biochemical_relationship","label":"CYP1A2 provides another route to the reactive intermediate.","description":"Recombinant human CYP1A2 catalyzed coumarin 3,4-epoxide formation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9fd9b93a-fdfd-5e09-92d3-0495bff41391","slug":"coumarin","display_name":"Coumarin","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9b15c13c-a369-5d10-a76a-525e8b19221d","slug":"cyp1a2","display_name":"Human cytochrome P450 1A2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"07724327-ab75-5ac3-94ae-00cb0577b4c5","slug":"coumarin-3-4-epoxide","display_name":"Coumarin 3,4-epoxide","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/coumarin-research/11950775.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"98a03fafa68ef4d55593a3b725af646175a11b42ce02b1dfe02be84ff49d4861\", \"start_char\": 0, \"end_char\": 1158, \"text_sha256\": \"98a03fafa68ef4d55593a3b725af646175a11b42ce02b1dfe02be84ff49d4861\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant CYP enzymes, immunoinhibition and mouse lung inhibitor experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"CYP1A/2E antibody inhibition and 5-phenyl-pentyne lung experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Relative contributions depend on species and tissue; this is not a clinical drug-interaction study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Coumarin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"coumarin","display_name":"Coumarin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human, rat and mouse; each claim specifies enzyme origin","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"CYP1A2 provides another route to the reactive intermediate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[coumarin-p11950775] Identification of the cytochromes P450 that catalyze coumarin 3,4-epoxidation and 3-hydroxylation. 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