{"id":"b8028961-e696-5c04-a1ce-053743882bd8","stable_key":"16c35a72-8162-5bb1-ae01-45331b8b42d0:moringa-cannabidiol-trpv1-separation","predicate":"contributes_to","statement":"A TRPV1 antagonist impaired cannabidiol efficacy in the macrophage assay but did not alter moringin activity; CB1 and CB2 antagonists affected neither route.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6dee0518-5e5d-5b0d-b2f1-7bb94cd28cd4","mechanism_event_label":"A TRPV1 antagonist impaired cannabidiol efficacy in the macrophage assay but did not alter moringin activity; CB1 and CB2 antagonists affected neither route.","subject":{"id":"8e0c61d8-3930-51d5-bce2-25a5edfb797c","slug":"human-trpv1","display_name":"Human transient receptor potential vanilloid 1 / TRPV1","entity_type_key":"protein"},"object":{"id":"7ea260ec-9c7e-5243-ab2a-01dec73027aa","slug":"cannabidiol-but-not-moringin-macrophage-efficacy","display_name":"TRPV1-sensitive cannabidiol efficacy but TRPV1-insensitive moringin efficacy in macrophages","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6dee0518-5e5d-5b0d-b2f1-7bb94cd28cd4","stable_key":"16c35a72-8162-5bb1-ae01-45331b8b42d0:moringa-cannabidiol-trpv1-separation-event","event_type":"observed_relationship","label":"A TRPV1 antagonist impaired cannabidiol efficacy in the macrophage assay but did not alter moringin activity; CB1 and CB2 antagonists affected neither route.","description":"A TRPV1 antagonist impaired cannabidiol efficacy in the macrophage assay but did not alter moringin activity; CB1 and CB2 antagonists affected neither route.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8e0c61d8-3930-51d5-bce2-25a5edfb797c","slug":"human-trpv1","display_name":"Human transient receptor potential vanilloid 1 / TRPV1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7ea260ec-9c7e-5243-ab2a-01dec73027aa","slug":"cannabidiol-but-not-moringin-macrophage-efficacy","display_name":"TRPV1-sensitive cannabidiol efficacy but TRPV1-insensitive moringin efficacy in macrophages","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"41236b3d-2b8f-5030-ab99-5a4a7cfed933","slug":"cannabidiol","display_name":"Cannabidiol","entity_type_key":"drug"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"18d01b02-1f9f-5dfd-8df2-a27c1899fa9c","slug":"moringin","display_name":"Moringin / MIC-1","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Cannabidiol or moringin with TRPV1, CB1 or CB2 antagonists","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. 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(2016). https://pubmed.ncbi.nlm.nih.gov/27215129/ DOI: 10.1016/j.fitote.2016.05.008","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro perturbation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Receptor-antagonist separation of combination components","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"009b3c0e-c682-592d-b32c-7cf78b843019","evidence_kind":"source_excerpt","locator":"Lines 101-110","start_line":101,"end_line":110,"excerpt":"## moringa-cannabidiol-trpv1-separation\nA TRPV1 antagonist impaired cannabidiol efficacy in the macrophage assay but did not alter moringin activity; CB1 and CB2 antagonists affected neither route.\nModel/species: LPS-stimulated murine macrophages\nTissue/system: Receptor-antagonist separation of combination components\nExposure: Cannabidiol or moringin with TRPV1, CB1 or CB2 antagonists\nRoute: In vitro perturbation\nDuration: Acute\nLimits: The abstract does not establish direct binding of either compound to every tested receptor, and the result is not a clinical interaction.\nPrimary reference: Anti-inflammatory and antioxidant effects of a combination of cannabidiol and moringin in LPS-stimulated macrophages. (2016). https://pubmed.ncbi.nlm.nih.gov/27215129/ DOI: 10.1016/j.fitote.2016.05.008\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"LPS-stimulated murine macrophages","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2752e9b3-e4b9-55fe-be07-944204cca4b8","stable_key":"import-16c35a72-8162-5bb1-ae01-45331b8b42d0","title":"Moringa oleifera: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. 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