{"id":"b7f331d8-51c4-52dc-aeb4-ef33feab94bc","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-wnk1-osmotic","predicate":"opposes_osmotic_activation","statement":"Chloride opposed osmolyte-stimulated WNK1 autophosphorylation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"dcce9e84-cffb-5cec-8fb4-e95acb616a53","mechanism_event_label":"Water balance and chloride jointly influence the kinase.","subject":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"object":{"id":"ba9aabe0-e3e5-5b4e-b4fc-be0d6b53bb7c","slug":"wnk1","display_name":"Human WNK lysine deficient protein kinase 1 / WNK1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"dcce9e84-cffb-5cec-8fb4-e95acb616a53","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-wnk1-osmotic-event","event_type":"biochemical_relationship","label":"Water balance and chloride jointly influence the kinase.","description":"Chloride opposed osmolyte-stimulated WNK1 autophosphorylation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ba9aabe0-e3e5-5b4e-b4fc-be0d6b53bb7c","slug":"wnk1","display_name":"Human WNK lysine deficient protein kinase 1 / WNK1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chloride-research/33689398.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"8a51e7a42ab9c614abf2c38e22f5d563d5416fffa11b7aec5919ee221a94e1b9\", \"start_char\": 0, \"end_char\": 1308, \"text_sha256\": \"8a51e7a42ab9c614abf2c38e22f5d563d5416fffa11b7aec5919ee221a94e1b9\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Biochemistry and structural measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"PEG400 or ethylene glycol osmotic challenge","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Artificial osmolytes; supports additional regulation, not a universal chloride-only switch.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chloride research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chloride","display_name":"Chloride","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human WNK1 and WNK3 constructs","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Water balance and chloride jointly influence the kinase.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chloride-p33689398] Osmosensing by WNK Kinases. 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