{"id":"b7d19c0e-1f0c-5a2b-afae-ba8e0e760bc6","stable_key":"1bbf9d44-8d2b-5b35-8f82-f6876558b0b3:cyclosporine-binds-mitochondrial-cyclophilin","predicate":"binds","statement":"Liver and heart mitochondrial matrix fractions, prepared free of membrane and cytosolic contamination, contain cyclosporin-sensitive peptidyl-prolyl cis-trans isomerase, titrated at 110.6 plus or minus 10.1 and 165.4 plus or minus 15.0 picomoles of enzyme per milligram of protein with a Ki of about 2.5 nanomolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a14172a2-0b68-5039-abf6-67921f83bd1b","mechanism_event_label":"Liver and heart mitochondrial matrix fractions, prepared free of membrane and cytosolic contamination, contain cyclosporin-sensitive peptidyl-prolyl cis-trans isomerase, titrated at 110.6 plus or minu","subject":{"id":"b199063a-fae6-5c5c-9cb4-22c10453bbac","slug":"cyclosporine","display_name":"Cyclosporine","entity_type_key":"drug"},"object":{"id":"ffae2606-e247-58de-9fed-16f62408bca2","slug":"mitochondrial-matrix-prolyl-isomerase","display_name":"Cyclosporin-sensitive mitochondrial matrix peptidyl-prolyl isomerase, 1990 titration","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"a14172a2-0b68-5039-abf6-67921f83bd1b","stable_key":"1bbf9d44-8d2b-5b35-8f82-f6876558b0b3:cyclosporine-binds-mitochondrial-cyclophilin-event","event_type":"biochemical_relationship","label":"Liver and heart mitochondrial matrix fractions, prepared free of membrane and cytosolic contamination, contain cyclosporin-sensitive peptidyl-prolyl cis-trans isomerase, titrated at 110.6 plus or minu","description":"Mitochondrial matrix peptidyl-prolyl isomerase","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b199063a-fae6-5c5c-9cb4-22c10453bbac","slug":"cyclosporine","display_name":"Cyclosporine","entity_type_key":"drug"},"role":"acting component","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ffae2606-e247-58de-9fed-16f62408bca2","slug":"mitochondrial-matrix-prolyl-isomerase","display_name":"Cyclosporin-sensitive mitochondrial matrix peptidyl-prolyl isomerase, 1990 titration","entity_type_key":"protein"},"role":"component the finding is about","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat liver and heart mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Cyclosporin A titration","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is a different cyclophilin from the cytosolic one that carries the immunosuppressive mechanism, and the two must not be merged. The 1990 report identifies a matrix isomerase by activity and binding site number; the gene assignment to Ppif came later and is not part of this measurement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Rat liver and heart mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Liver and heart mitochondrial matrix fractions, prepared free of membrane and cytosolic contamination, contain cyclosporin-sensitive peptidyl-prolyl cis-trans isomerase, titrated at 110.6 plus or minus 10.1 and 165.4 plus or minus 15.0 picomoles of enzyme per milligram of protein with a Ki of about 2.5 nanomolar.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibition of Ca2+-induced large-amplitude swelling of liver and heart mitochondria by cyclosporin is probably caused by the inhibitor binding to mitochondrial-matrix peptidyl-prolyl cis-trans isomerase and preventing it interacting with the adenine nucleotide translocase. (1990). https://pubmed.ncbi.nlm.nih.gov/2160810/ DOI: 10.1042/bj2680153","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Mitochondrial matrix peptidyl-prolyl isomerase","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4ee2e998-85a2-5a74-8b9f-9935e33ca744","evidence_kind":"source_excerpt","locator":"Lines 124-124","start_line":124,"end_line":124,"excerpt":"Liver and heart mitochondrial matrix fractions, prepared free of membrane and cytosolic contamination, contain cyclosporin-sensitive peptidyl-prolyl cis-trans isomerase, titrated at 110.6 plus or minus 10.1 and 165.4 plus or minus 15.0 picomoles of enzyme per milligram of protein with a Ki of about 2.5 nanomolar.","model_system":"Rat liver and heart mitochondria","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"6b2b7746-fc66-5491-b896-e6261df254c9","stable_key":"import-1bbf9d44-8d2b-5b35-8f82-f6876558b0b3","title":"Cyclosporine: the complex that inhibits calcineurin, a second cyclophilin, and the transport step that decides exposure (2026-09-23)","document_type":"imported_text","citation_label":"Original AI-assisted curation of seven primary studies resolved by PubMed title search, with every abstract read and all DOIs cross-checked against live PubMed metadata on 2026-09-23. No reference carries a recorded retraction, erratum or expression of concern. Each of the seven is a separate laboratory and each carries its own lineage key, so none of them can be counted twice as independent support. Study-specific concentrations, kinetic constants and limitations retained. Not publisher full text.","file_path":"","sha256":"499d00555a629cba2271c3888d7e93627949891d565c4c78c843610de2913080","revision_id":"263ee68a-4184-51d0-a977-197d7ddb7a4d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}