{"id":"b7cc0635-e4d7-544f-9f46-ba0dc9852b9b","stable_key":"8c946991-97f0-55ee-8df0-00e67621962f:nad-plus-treg-death","predicate":"mediates","statement":"NAD administration depleted mouse regulatory T cells through the ART2.2/P2X7 pathway; ART2.2-blocking antibody protected against the effect.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c38edfb0-85a7-52bd-b95a-034012a41633","mechanism_event_label":"A receptor-linked extracellular reaction can determine which immune cells survive.","subject":{"id":"5debceb9-d82b-5648-b271-807ad43ff66a","slug":"mouse-p2rx7","display_name":"Mouse purinergic receptor P2X7 / P2rx7","entity_type_key":"protein"},"object":{"id":"9a67d370-5f7c-5478-9ded-e4aa03ad50aa","slug":"mouse-nad-induced-treg-death","display_name":"NAD-induced mouse regulatory T-cell death","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c38edfb0-85a7-52bd-b95a-034012a41633","stable_key":"8c946991-97f0-55ee-8df0-00e67621962f:nad-plus-treg-death-event","event_type":"observed_relationship","label":"A receptor-linked extracellular reaction can determine which immune cells survive.","description":"NAD administration depleted mouse regulatory T cells through the ART2.2/P2X7 pathway; ART2.2-blocking antibody protected against the effect.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5debceb9-d82b-5648-b271-807ad43ff66a","slug":"mouse-p2rx7","display_name":"Mouse purinergic receptor P2X7 / P2rx7","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9a67d370-5f7c-5478-9ded-e4aa03ad50aa","slug":"mouse-nad-induced-treg-death","display_name":"NAD-induced mouse regulatory T-cell death","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b8cd8c8d-7836-5636-9b3b-35e4a59084a2","slug":"mouse-art2b","display_name":"Mouse ADP-ribosyltransferase ARTC2.2 / Art2b","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse in-vitro and in-vivo NAD intervention.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Species and exposure dependent; cannot be directly assigned to human regulatory T cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"NAD+ collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A receptor-linked extracellular reaction can determine which immune cells survive.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Extracellular NAD+ shapes the Foxp3+ regulatory T cell compartment through the ART2-P2X7 pathway. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20975043/ · DOI 10.1084/jem.20091154","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c0ca56ff-deb9-54c0-a303-f563382a7ea2","evidence_kind":"source_excerpt","locator":"Lines 276-282","start_line":276,"end_line":282,"excerpt":"## nad-plus-treg-death\nA receptor-linked extracellular reaction can determine which immune cells survive.\nNAD administration depleted mouse regulatory T cells through the ART2.2/P2X7 pathway; ART2.2-blocking antibody protected against the effect.\nModel: Mouse in-vitro and in-vivo NAD intervention.\nLimitations: Species and exposure dependent; cannot be directly assigned to human regulatory T cells.\nEvidence access: Primary full text\nExtracellular NAD+ shapes the Foxp3+ regulatory T cell compartment through the ART2-P2X7 pathway. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20975043/ · DOI 10.1084/jem.20091154","model_system":"Mouse in-vitro and in-vivo NAD intervention.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f59aa302-d463-5462-876f-e21e64861eac","stable_key":"import-8c946991-97f0-55ee-8df0-00e67621962f","title":"NAD+: compartmental supply, consumption and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"9f5324d0d652c80ad630b3100e28ce34cd2ce260ea2e1324332a4262ce72ae09","revision_id":"0e07909c-e8bc-5950-bafc-61371d9d6412","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}