{"id":"b7a6d374-e736-5a6c-9440-ff13d73b58f4","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:mouse-dlat-hadha-association","predicate":"associates_with","statement":"Mouse cardiac Dlat and HADHA co-immunoprecipitated; their association was stronger in the HFpEF model.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2fefc510-bf84-57eb-aa94-c581ce3445b9","mechanism_event_label":"mouse dlat hadha association","subject":{"id":"80e2b56a-67d4-54a2-a847-2f55159ce64d","slug":"mouse-dlat","display_name":"Mouse dihydrolipoyl acetyltransferase / Dlat","entity_type_key":"protein"},"object":{"id":"0c45e35a-9af8-52e0-ac32-fde170ef9158","slug":"mouse-hadha","display_name":"Mouse mitochondrial trifunctional protein alpha subunit / Hadha","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"2fefc510-bf84-57eb-aa94-c581ce3445b9","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:mouse-dlat-hadha-association","event_type":"associates_with","label":"mouse dlat hadha association","description":"Model-specific finding; read linked claim context and source-access tier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80e2b56a-67d4-54a2-a847-2f55159ce64d","slug":"mouse-dlat","display_name":"Mouse dihydrolipoyl acetyltransferase / Dlat","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0c45e35a-9af8-52e0-ac32-fde170ef9158","slug":"mouse-hadha","display_name":"Mouse mitochondrial trifunctional protein alpha subunit / Hadha","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"full_text_and_supplement_review","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"compartment","value_text":"Cardiac tissue / mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Two-hit model: 60% fat-calorie diet and L-NAME 0.5 g/L drinking water; vector dose not reported in reviewed text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Two-hit protocol 15 weeks. Transgenic age/induction wording differs across passages; do not infer one exact interval","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"mouse-hadha","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Reciprocal co-immunoprecipitation; Figure 6D/E/H","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse two-hit HFpEF model: 60% fat-calorie diet plus L-NAME 0.5 g/L drinking water; additional intervention as stated","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"mouse-dlat","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Co-immunoprecipitation establishes association, not by itself direct binding or acetyl transfer.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Mouse cardiac Dlat and HADHA co-immunoprecipitated; their association was stronger in the HFpEF model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"[{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 44, \"text_sha256\": \"e0a92f47bc76c894816706ffb5158242c968928ac61e4561262c370035eda621\", \"xml_element_id\": \"Par19\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 46, \"text_sha256\": \"d56a90702bdf004de1efc2e43103b1b771e15b38288dc01f3741676782b1f5ff\", \"xml_element_id\": null}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-026-70703-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"sample_size","value_text":"6 per group","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cardiac tissue and cardiomyocytes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"748aebfa-a900-5d94-8c5f-c53ab487f2d9","evidence_kind":"source_excerpt","locator":"Lines 16-31","start_line":16,"end_line":31,"excerpt":"Mouse cardiac Dlat and HADHA co-immunoprecipitated; their association was stronger in the HFpEF model.\norganism: Mus musculus\ntissue_or_cell_type: Cardiac tissue and cardiomyocytes\nexperimental_model: Mouse two-hit HFpEF model: 60% fat-calorie diet plus L-NAME 0.5 g/L drinking water; additional intervention as stated\ncompartment: Cardiac tissue / mitochondria\ndose: Two-hit model: 60% fat-calorie diet and L-NAME 0.5 g/L drinking water; vector dose not reported in reviewed text\nduration: Two-hit protocol 15 weeks. Transgenic age/induction wording differs across passages; do not infer one exact interval\nprimary_references: https://doi.org/10.1038/s41467-026-70703-w\naccess_level: full_text_and_supplement_review\nevidence_location: Reciprocal co-immunoprecipitation; Figure 6D/E/H\nendpoint: mouse-hadha\nexposure: mouse-dlat\nlimitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Co-immunoprecipitation establishes association, not by itself direct binding or acetyl transfer.\nprimary_locator: [{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 44, \"text_sha256\": \"e0a92f47bc76c894816706ffb5158242c968928ac61e4561262c370035eda621\", \"xml_element_id\": \"Par19\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 46, \"text_sha256\": \"d56a90702bdf004de1efc2e43103b1b771e15b38288dc01f3741676782b1f5ff\", \"xml_element_id\": null}]\nplain_language: Mouse cardiac Dlat and HADHA co-immunoprecipitated; their association was stronger in the HFpEF model.\nsample_size: 6 per group","model_system":"Mouse two-hit HFpEF model: 60% fat-calorie diet plus L-NAME 0.5 g/L drinking water; additional intervention as stated","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Quotation is the authored curation, not original publisher wording. Exact primary locators and access limits retained in contexts.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ee72956d-4fd4-58d3-9a94-e173bb1e3282","stable_key":"import-c156003d-8cdf-5259-8561-9803795ecc82","title":"DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence","document_type":"imported_text","citation_label":"AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation.","file_path":"","sha256":"159e5472c8b1d104625f9c648dc9ee24dcd5fe102109fd960bba50e9ba9de954","revision_id":"5ca6b1f1-fd75-5f8d-ba2b-0215fbf387c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}