{"id":"b768e870-44f2-5ed3-95ae-621bed889f11","stable_key":"7484635e-cc8f-5cd6-a978-6dc5775a7db5:berberine-kcnq1-current","predicate":"inhibits","statement":"Berberine inhibited a cAMP-dependent, chromanol-sensitive basolateral potassium current consistent with KCNQ1 by 88%.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ab3c49a0-32e9-53c5-aba8-57f0012506b4","mechanism_event_label":"Reducing potassium recycling can limit chloride secretion across intestinal cells.","subject":{"id":"7bbe0633-b2f1-510f-adc0-3c4d0ddad05d","slug":"berberine","display_name":"Berberine","entity_type_key":"small_molecule"},"object":{"id":"bc2b59c6-c9f2-5b9b-a7b6-549201dd8c6b","slug":"kcnq1-basolateral-potassium-current","display_name":"Basolateral KCNQ1-associated potassium current","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ab3c49a0-32e9-53c5-aba8-57f0012506b4","stable_key":"7484635e-cc8f-5cd6-a978-6dc5775a7db5:berberine-kcnq1-current-event","event_type":"biochemical_relationship","label":"Reducing potassium recycling can limit chloride secretion across intestinal cells.","description":"Berberine inhibited a cAMP-dependent, chromanol-sensitive basolateral potassium current consistent with KCNQ1 by 88%.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7621dbe0-e1dc-5d55-b206-56820074c8ac","slug":"kcnq1","display_name":"Human KCNQ1 / Kv7.1 potassium channel","entity_type_key":"protein"},"role":"implicated_channel","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"role":"transported_ion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7bbe0633-b2f1-510f-adc0-3c4d0ddad05d","slug":"berberine","display_name":"Berberine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bc2b59c6-c9f2-5b9b-a7b6-549201dd8c6b","slug":"kcnq1-basolateral-potassium-current","display_name":"Basolateral KCNQ1-associated potassium current","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/berberine-research/21747769.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b8864a2844ea828aeabcfc05219050ac1cb6efe7f24c663dc3302887f7f59ad4\", \"start_char\": 0, \"end_char\": 1734, \"text_sha256\": \"b8864a2844ea828aeabcfc05219050ac1cb6efe7f24c663dc3302887f7f59ad4\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Ussing-chamber short-circuit current, patch clamp and kinase perturbation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Forskolin-stimulated secretion and berberine; IC50 about 80 micromolar","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Intestinal cell mechanism, not proof of systemic potassium depletion. The observed channel complex differs from cardiac KCNQ1/KCNE1.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Berberine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"berberine","display_name":"Berberine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human T84 colonic cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Reducing potassium recycling can limit chloride secretion across intestinal cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[berberine-p21747769] Berberine Reduces cAMP-Induced Chloride Secretion in T84 Human Colonic Carcinoma Cells through Inhibition of Basolateral KCNQ1 Channels. (2011). https://pubmed.ncbi.nlm.nih.gov/21747769/ DOI: 10.3389/fphys.2011.00033","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Basolateral potassium recycling and chloride secretion","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4bd30fcd-8e7c-5c4c-81a5-1e09289dfb0d","evidence_kind":"source_excerpt","locator":"Lines 558-569","start_line":558,"end_line":569,"excerpt":"### berberine-kcnq1-current\nBerberine inhibited a cAMP-dependent, chromanol-sensitive basolateral potassium current consistent with KCNQ1 by 88%.\nCondition category: normal\nnutrient_topic: Berberine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Reducing potassium recycling can limit chloride secretion across intestinal cells.\norganism: Human T84 colonic cells\ntissue_or_cell_type: Basolateral potassium recycling and chloride secretion\nexperimental_model: Ussing-chamber short-circuit current, patch clamp and kinase perturbation\nlimitations: Intestinal cell mechanism, not proof of systemic potassium depletion. The observed channel complex differs from cardiac KCNQ1/KCNE1.\nexposure: Forskolin-stimulated secretion and berberine; IC50 about 80 micromolar\nevidence_span: {\"source_cache\": \"artifacts/berberine-research/21747769.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b8864a2844ea828aeabcfc05219050ac1cb6efe7f24c663dc3302887f7f59ad4\", \"start_char\": 0, \"end_char\": 1734, \"text_sha256\": \"b8864a2844ea828aeabcfc05219050ac1cb6efe7f24c663dc3302887f7f59ad4\"}\n[berberine-p21747769] Berberine Reduces cAMP-Induced Chloride Secretion in T84 Human Colonic Carcinoma Cells through Inhibition of Basolateral KCNQ1 Channels. 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