{"id":"b75365ec-d6d0-58f9-9293-76c3e2ea99e8","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-tlr-mycobacteria","predicate":"activation-reduces-intracellular-viability","statement":"TLR activation coupled vitamin D pathway induction to cathelicidin expression and killing of intracellular M. tuberculosis in the human macrophage experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b06ed3aa-ebb6-5cb2-a6f8-ef89e4a4890b","mechanism_event_label":"The cell experiment linked local vitamin D signaling to bacterial killing.","subject":{"id":"d7edcae6-c1e9-5013-a99e-002acc62eef8","slug":"tlr2-tlr1","display_name":"TLR2-TLR1 receptor complex","entity_type_key":"protein_complex"},"object":{"id":"8cfa6b7b-c815-5dc1-9200-b7abcf848b4b","slug":"intracellular-mycobacterial-viability","display_name":"Intracellular Mycobacterium tuberculosis viability","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b06ed3aa-ebb6-5cb2-a6f8-ef89e4a4890b","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-tlr-mycobacteria-event","event_type":"biochemical_relationship","label":"The cell experiment linked local vitamin D signaling to bacterial killing.","description":"TLR activation coupled vitamin D pathway induction to cathelicidin expression and killing of intracellular M. tuberculosis in the human macrophage experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6bd73bd2-1f0a-5caa-ba69-7ce1b75364bb","slug":"calcifediol","display_name":"Calcifediol / 25-hydroxyvitamin D3","entity_type_key":"small_molecule"},"role":"precursor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f5156c24-108f-5746-b114-ed764e79a3f4","slug":"calcitriol","display_name":"Calcitriol","entity_type_key":"small_molecule"},"role":"active-metabolite","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"098c0ca2-40c4-59d1-ad04-a7e14b80be50","slug":"human-cathelicidin-precursor","display_name":"Human cathelicidin precursor hCAP18","entity_type_key":"protein"},"role":"antimicrobial-pathway","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"d7edcae6-c1e9-5013-a99e-002acc62eef8","slug":"tlr2-tlr1","display_name":"TLR2-TLR1 receptor complex","entity_type_key":"protein_complex"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"8cfa6b7b-c815-5dc1-9200-b7abcf848b4b","slug":"intracellular-mycobacterial-viability","display_name":"Intracellular Mycobacterium tuberculosis viability","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human monocyte/macrophage TLR stimulation and intracellular mycobacterial assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Cultured human macrophages with intracellular M. tuberculosis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cellular experimental result, not a human tuberculosis-treatment trial; infection response depends on more than vitamin D.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The cell experiment linked local vitamin D signaling to bacterial killing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[liu2006] Toll-like receptor triggering of a vitamin D-mediated human antimicrobial response. (2006). https://pubmed.ncbi.nlm.nih.gov/16497887/ DOI: 10.1126/science.1123933","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Human macrophages","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c5611f34-e55d-562f-9e2e-2a14eb2e7e82","evidence_kind":"source_excerpt","locator":"Lines 970-981","start_line":970,"end_line":981,"excerpt":"### vd-tlr-mycobacteria\nTLR activation coupled vitamin D pathway induction to cathelicidin expression and killing of intracellular M. tuberculosis in the human macrophage experiments.\nCondition category: normal\nnutrient_topic: Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The cell experiment linked local vitamin D signaling to bacterial killing.\norganism: Homo sapiens\ntissue_or_cell_type: Human macrophages\nexperimental_model: Human monocyte/macrophage TLR stimulation and intracellular mycobacterial assay\nlimitations: Cellular experimental result, not a human tuberculosis-treatment trial; infection response depends on more than vitamin D.\nexposure: Cultured human macrophages with intracellular M. tuberculosis.\ncross_nutrient: false\n[liu2006] Toll-like receptor triggering of a vitamin D-mediated human antimicrobial response. 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