{"id":"b702691a-b6b8-5099-9f00-adad502c2040","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-mouse-tdh-flux","predicate":"supports","statement":"Mouse embryonic stem cells expressed abundant Tdh and used mitochondrial threonine catabolism to support glycine and acetyl-CoA generation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ac641790-9cc4-5470-be16-9c7f5f169a74","mechanism_event_label":"A specialized mouse cell uses threonine as both carbon and one-carbon support.","subject":{"id":"9b018d12-5ae6-5c78-bd51-84c2bd7f18a7","slug":"mouse-tdh","display_name":"Mouse L-threonine dehydrogenase / Tdh","entity_type_key":"protein"},"object":{"id":"3e7cf137-13f1-55fa-8718-ce6d93ca6085","slug":"mouse-threonine-glycine-acetyl-coa","display_name":"Mouse embryonic stem-cell threonine-derived glycine and acetyl-CoA","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ac641790-9cc4-5470-be16-9c7f5f169a74","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-mouse-tdh-flux-event","event_type":"observed_relationship","label":"A specialized mouse cell uses threonine as both carbon and one-carbon support.","description":"Mouse embryonic stem cells expressed abundant Tdh and used mitochondrial threonine catabolism to support glycine and acetyl-CoA generation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9b018d12-5ae6-5c78-bd51-84c2bd7f18a7","slug":"mouse-tdh","display_name":"Mouse L-threonine dehydrogenase / Tdh","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3e7cf137-13f1-55fa-8718-ce6d93ca6085","slug":"mouse-threonine-glycine-acetyl-coa","display_name":"Mouse embryonic stem-cell threonine-derived glycine and acetyl-CoA","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse embryonic stem-cell metabolism and amino-acid withdrawal experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Canonical human TDH is nonfunctional; this route is explicitly mouse-specific.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Threonine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A specialized mouse cell uses threonine as both carbon and one-carbon support.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Dependence of mouse embryonic stem cells on threonine catabolism. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19589965/ · DOI 10.1126/science.1173288","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ae828683-2be1-50f1-8dd4-4ab2a479d01f","evidence_kind":"source_excerpt","locator":"Lines 322-328","start_line":322,"end_line":328,"excerpt":"## l-threonine-mouse-tdh-flux\nA specialized mouse cell uses threonine as both carbon and one-carbon support.\nMouse embryonic stem cells expressed abundant Tdh and used mitochondrial threonine catabolism to support glycine and acetyl-CoA generation.\nModel: Mouse embryonic stem-cell metabolism and amino-acid withdrawal experiments.\nLimitations: Canonical human TDH is nonfunctional; this route is explicitly mouse-specific.\nEvidence access: Primary abstract\nDependence of mouse embryonic stem cells on threonine catabolism. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19589965/ · DOI 10.1126/science.1173288","model_system":"Mouse embryonic stem-cell metabolism and amino-acid withdrawal experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"35396730-235a-537e-8a05-c6ef2960c509","stable_key":"import-97957230-601f-5dec-8524-0812be8fadbf","title":"L-Threonine: translation, intestinal barrier, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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