{"id":"b6ee9bc2-0ef4-5eee-8967-75ef055521f7","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-txnrd-active-site","predicate":"modifies_reduced_active_site","statement":"Myricetin inhibition was time- and NADPH-dependent and involved the reduced C-terminal Cys-Sec-Gly active site.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a5d3266c-d6e8-5957-a82f-f700e01dab1b","mechanism_event_label":"The enzyme’s chemical state affects vulnerability.","subject":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"object":{"id":"44c93642-76cb-5254-9edb-14a3020a5ae6","slug":"rat-txnrd1","display_name":"Rat thioredoxin reductase 1 / Txnrd1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"a5d3266c-d6e8-5957-a82f-f700e01dab1b","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-txnrd-active-site-event","event_type":"observed_relationship","label":"The enzyme’s chemical state affects vulnerability.","description":"Myricetin inhibition was time- and NADPH-dependent and involved the reduced C-terminal Cys-Sec-Gly active site.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"44c93642-76cb-5254-9edb-14a3020a5ae6","slug":"rat-txnrd1","display_name":"Rat thioredoxin reductase 1 / Txnrd1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4aba2a5e-8d06-5304-bb01-c0402b225a94","slug":"nadph","display_name":"NADPH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"42620d13-f86a-5e08-acea-7bd24787cbe4","slug":"protein-incorporated-selenocysteine","display_name":"Protein-incorporated selenocysteine residue","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract and publisher methods/results","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat TrxR biochemical experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Semiquinone mediation was proposed; do not treat the precise intermediate as directly proven.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Myricetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The enzyme’s chemical state affects vulnerability.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibition of Mammalian thioredoxin reductase by some flavonoids: implications for myricetin and quercetin anticancer activity. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16618767/ · DOI 10.1158/0008-5472.CAN-05-3310","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1d08958d-adb9-5b82-aee3-52b8c56af5c0","evidence_kind":"source_excerpt","locator":"Lines 260-266","start_line":260,"end_line":266,"excerpt":"## myricetin-txnrd-active-site\nThe enzyme’s chemical state affects vulnerability.\nMyricetin inhibition was time- and NADPH-dependent and involved the reduced C-terminal Cys-Sec-Gly active site.\nModel: Rat TrxR biochemical experiments.\nLimitations: Semiquinone mediation was proposed; do not treat the precise intermediate as directly proven.\nEvidence access: Primary abstract and publisher methods/results\nInhibition of Mammalian thioredoxin reductase by some flavonoids: implications for myricetin and quercetin anticancer activity. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16618767/ · DOI 10.1158/0008-5472.CAN-05-3310","model_system":"Rat TrxR biochemical experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"58045cd9-3b27-5469-bb4b-e1c6a9bdca34","stable_key":"import-8182578e-8d21-598e-a3cb-e62d480d2251","title":"Myricetin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"0abf512275cf3aae9e4ec8544f1cde97e0155d0cfb5be12a193cdd5a898386a7","revision_id":"422e342f-a55c-5e73-ba98-43df560f49b9","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}