{"id":"b6b62ec2-e682-5b81-ba02-9c41f0851c8f","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-methyl-folate-sam-bhmt","predicate":"increases","statement":"The 20 mg folate/kg diet increased hepatic SAM in Bhmt-null mice versus 0 and 2 mg/kg diets.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"fd950353-deb9-5d39-8892-952a854da663","mechanism_event_label":"SAM improved despite persistent high homocysteine.","subject":{"id":"d322bfef-5135-599f-82e6-0aa58748dc8b","slug":"folic-acid","display_name":"Folic acid","entity_type_key":"small_molecule"},"object":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"fd950353-deb9-5d39-8892-952a854da663","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-methyl-folate-sam-bhmt-event","event_type":"biochemical_relationship","label":"SAM improved despite persistent high homocysteine.","description":"The 20 mg folate/kg diet increased hepatic SAM in Bhmt-null mice versus 0 and 2 mg/kg diets.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d322bfef-5135-599f-82e6-0aa58748dc8b","slug":"folic-acid","display_name":"Folic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"Bhmt-null and wild-type mice; four-week folate feeding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Four-week feeding","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No proof of restored DNA methylation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"SAM improved despite persistent high homocysteine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[teng-2012] Homocysteinemia in mice with genetic betaine homocysteine S-methyltransferase deficiency is independent of dietary folate intake (2012). https://pubmed.ncbi.nlm.nih.gov/23014492/ DOI: 10.3945/jn.112.166835","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Liver","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"0d7381bb-f971-5415-b8de-7582b6309977","evidence_kind":"source_excerpt","locator":"Lines 681-691","start_line":681,"end_line":691,"excerpt":"### folate-methyl-folate-sam-bhmt\nThe 20 mg folate/kg diet increased hepatic SAM in Bhmt-null mice versus 0 and 2 mg/kg diets.\nCondition category: machinery_impairment\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: SAM improved despite persistent high homocysteine.\norganism: Mus musculus\ntissue_or_cell_type: Liver\nexperimental_model: Bhmt-null and wild-type mice; four-week folate feeding.\nlimitations: No proof of restored DNA methylation.\nexposure: Four-week feeding\n[teng-2012] Homocysteinemia in mice with genetic betaine homocysteine S-methyltransferase deficiency is independent of dietary folate intake (2012). https://pubmed.ncbi.nlm.nih.gov/23014492/ DOI: 10.3945/jn.112.166835","model_system":"Bhmt-null and wild-type mice; four-week folate feeding.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [teng-2012] Homocysteinemia in mice with genetic betaine homocysteine S-methyltransferase deficiency is independent of dietary folate intake (2012). https://pubmed.ncbi.nlm.nih.gov/23014492/ DOI: 10.3945/jn.112.166835","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}