{"id":"b64494fa-d9b8-5847-a99a-d7f06d311f57","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:mtt-null","predicate":"no_detected_change_in_recorded_experiment","statement":"MTT metabolic-activity readout did not significantly decrease after exposure up to 100 uM in the tested Jurkat system.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"da90ce7a-4d48-59f3-9d9c-6d788d88cff0","mechanism_event_label":"MTT metabolic-activity readout did not significantly decrease after exposure up to 100 uM in the tested Jurkat system.","subject":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"object":{"id":"5a21f716-c22c-5445-b63f-767be1f01542","slug":"human-jurkat-mtt-activity","display_name":"MTT metabolic-activity readout in human Jurkat cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"da90ce7a-4d48-59f3-9d9c-6d788d88cff0","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:mtt-null-event","event_type":"experimental_observation","label":"MTT metabolic-activity readout did not significantly decrease after exposure up to 100 uM in the tested Jurkat system.","description":"**The human protein experiment.** Direct exposure of Jurkat cells to 100 µM allicin for ten minutes produced S-thioallylated peptides from 332 proteins, detected by an approximately +72-Da cysteine modification. Total free thiols fell by about half. Targets reported in the main text include ALDOA, GAPDH, PKM, ENO1, SOD1, PRDX1, CFL1, LCP1, FLNA, FLNB, HSPA4, EEF2, and HMGB1, alongside actin, tubulin, and HSP90 groups. These are adduct records; individual isoforms are not invented where unresolved. ENO1-associated lysate activity decreased, while the twenty-four-hour MTT readout was unchanged at up to 100 µM. MTT preservation does not mean every cellular function was unaffected. [Gruhlke 2019](https://pmc.ncbi.nlm.nih.gov/articles/PMC6342545/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Allicin exposure at the specified assay dose/time","sequence_order":0,"notes":""},{"entity":{"id":"5a21f716-c22c-5445-b63f-767be1f01542","slug":"human-jurkat-mtt-activity","display_name":"MTT metabolic-activity readout in human Jurkat cells","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"no_detected_change","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Allicin exposure at the specified assay dose/time\", \"comparator\": \"Untreated matched cells\", \"endpoint\": \"MTT metabolic-activity readout did not significantly decrease after exposure up to 100 uM in the tested Jurkat system.\", \"effect_direction\": \"no_detected_change\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Human Jurkat cells; ten-minute 100 uM exposure for thiols/enolase, 24-hour MTT observation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"MTT, free thiols and specific enzyme activity measure different quantities. The lysate assay does not isolate every possible contribution to inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"MTT metabolic-activity readout did not significantly decrease after exposure up to 100 uM in the tested Jurkat system.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The human allicin-proteome: S-thioallylation of proteins by the garlic defence substance allicin and its biological effects. | 2019 | DOI 10.1016/j.freeradbiomed.2018.11.022 | PMID 30500420 | https://pubmed.ncbi.nlm.nih.gov/30500420/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC6342545/ | https://doi.org/10.1016/j.freeradbiomed.2018.11.022","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 43-43; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"945fe6e6-1a12-5913-a3e5-398da366532d","evidence_kind":"source_excerpt","locator":"Lines 43-43","start_line":43,"end_line":43,"excerpt":"**The human protein experiment.** Direct exposure of Jurkat cells to 100 µM allicin for ten minutes produced S-thioallylated peptides from 332 proteins, detected by an approximately +72-Da cysteine modification. Total free thiols fell by about half. Targets reported in the main text include ALDOA, GAPDH, PKM, ENO1, SOD1, PRDX1, CFL1, LCP1, FLNA, FLNB, HSPA4, EEF2, and HMGB1, alongside actin, tubulin, and HSP90 groups. These are adduct records; individual isoforms are not invented where unresolved. ENO1-associated lysate activity decreased, while the twenty-four-hour MTT readout was unchanged at up to 100 µM. MTT preservation does not mean every cellular function was unaffected. [Gruhlke 2019](https://pmc.ncbi.nlm.nih.gov/articles/PMC6342545/)","model_system":"Human Jurkat cells; ten-minute 100 uM exposure for thiols/enolase, 24-hour MTT observation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"10820a6b-594a-547d-97f9-906ab4cc1d6e","stable_key":"import-c836a883-ac18-5eb2-9971-2f0b542feba8","title":"Allicin: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"2475d3eb681100a0a34577a47b7cba5b251df866fbd6ce795122ccabee360018","revision_id":"590df96d-2ed1-5763-b04c-bf0e096e603c","review_status":"unverified_draft","notes":""}}],"relations":[{"id":"95d0dfbc-7725-5005-941c-b010db269713","relation_type":"limits_scope_of","notes":"Protein modification and preserved MTT signal coexist; do not equate adducts with cell death.","source_claim_id":"b64494fa-d9b8-5847-a99a-d7f06d311f57","source_claim_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:mtt-null","source_statement":"MTT metabolic-activity readout did not significantly decrease after exposure up to 100 uM in the tested Jurkat system.","target_claim_id":"22f51955-2d08-598b-b42a-f12e0edc2115","target_claim_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:proteome-adducts","target_statement":"S-thioallylated peptides were detected from 332 proteins after direct allicin exposure."}],"conflicts":[],"corrections":[],"research":null}