{"id":"b63c4066-5f66-5553-9945-04dd541e5c90","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-chdh-aldehyde-step","predicate":"oxidizes_choline_to","statement":"The pathway model identifies CHDH-mediated oxidation of choline to betaine aldehyde upstream of aldehyde-dehydrogenase-mediated betaine production.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"714c8818-bc8a-5fa4-a6e0-329de1254f8b","mechanism_event_label":"Choline oxidation has an aldehyde intermediate; it is not one undifferentiated jump to betaine.","subject":{"id":"d28132cb-c5b1-5423-879b-fe68a93a8ba4","slug":"chdh","display_name":"Human choline dehydrogenase / CHDH","entity_type_key":"protein"},"object":{"id":"2922542d-d263-5f46-afde-503b30e4940d","slug":"betaine-aldehyde","display_name":"Betaine aldehyde","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"714c8818-bc8a-5fa4-a6e0-329de1254f8b","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-chdh-aldehyde-step-event","event_type":"biochemical_relationship","label":"Choline oxidation has an aldehyde intermediate; it is not one undifferentiated jump to betaine.","description":"The pathway model identifies CHDH-mediated oxidation of choline to betaine aldehyde upstream of aldehyde-dehydrogenase-mediated betaine production.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8e1d19fd-ad66-575d-81c4-9122f78915e5","slug":"choline","display_name":"Choline","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d28132cb-c5b1-5423-879b-fe68a93a8ba4","slug":"chdh","display_name":"Human choline dehydrogenase / CHDH","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2922542d-d263-5f46-afde-503b30e4940d","slug":"betaine-aldehyde","display_name":"Betaine aldehyde","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/choline-research/39111307.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"086a515ebf324a03baeb82e7fe21771eb7f819da09ef7a22372e06db25d3c571\", \"start_char\": 3335, \"end_char\": 3656, \"text_sha256\": \"580d72b54dac50ca5d1cfdb4e2ffe3719e957af76ac81d761422471424636d69\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Slc25a48 knockout, rescue, mitochondrial assays and isotope tracing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Biochemical pathway described in the study introduction","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Established pathway background in this carrier study, not a newly isolated human CHDH catalytic assay. No cofactor identity or exclusive downstream ALDH isoform is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Choline research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"choline","display_name":"Choline","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mammalian pathway background applied to the human-cell arm","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Choline oxidation has an aldehyde intermediate; it is not one undifferentiated jump to betaine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[choline-p39111307] SLC25A48 controls mitochondrial choline import and metabolism. (2024). https://pubmed.ncbi.nlm.nih.gov/39111307/ DOI: 10.1016/j.cmet.2024.07.010","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Mitochondrial choline metabolism","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"90a4254f-47e2-53eb-a325-4b01ad0e0168","evidence_kind":"source_excerpt","locator":"Lines 464-475","start_line":464,"end_line":475,"excerpt":"### choline-chdh-aldehyde-step\nThe pathway model identifies CHDH-mediated oxidation of choline to betaine aldehyde upstream of aldehyde-dehydrogenase-mediated betaine production.\nCondition category: normal\nnutrient_topic: Choline research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Choline oxidation has an aldehyde intermediate; it is not one undifferentiated jump to betaine.\norganism: Mammalian pathway background applied to the human-cell arm\ntissue_or_cell_type: Mitochondrial choline metabolism\nexperimental_model: Slc25a48 knockout, rescue, mitochondrial assays and isotope tracing\nlimitations: Established pathway background in this carrier study, not a newly isolated human CHDH catalytic assay. No cofactor identity or exclusive downstream ALDH isoform is inferred.\nexposure: Biochemical pathway described in the study introduction\nevidence_span: {\"source_cache\": \"artifacts/choline-research/39111307.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"086a515ebf324a03baeb82e7fe21771eb7f819da09ef7a22372e06db25d3c571\", \"start_char\": 3335, \"end_char\": 3656, \"text_sha256\": \"580d72b54dac50ca5d1cfdb4e2ffe3719e957af76ac81d761422471424636d69\"}\n[choline-p39111307] SLC25A48 controls mitochondrial choline import and metabolism. (2024). https://pubmed.ncbi.nlm.nih.gov/39111307/ DOI: 10.1016/j.cmet.2024.07.010","model_system":"Slc25a48 knockout, rescue, mitochondrial assays and isotope tracing","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [choline-p39111307] SLC25A48 controls mitochondrial choline import and metabolism. (2024). https://pubmed.ncbi.nlm.nih.gov/39111307/ DOI: 10.1016/j.cmet.2024.07.010","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0ba2f05e-5cf5-5bd7-ae5d-0ab4945fcb7b","stable_key":"import-27e0c1cf-7726-5164-8b15-27a631584cd0","title":"Choline: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"c7afe3d579eba0df6d2a4c26699c653462749e03188348f900c07748cc7282a8","revision_id":"0fa7b48f-2a3c-5adc-b13e-48727dd6f815","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}