{"id":"b6378135-34cc-50db-947c-02bb8a53e603","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-nrf2-location","predicate":"promoted","statement":"Carotenoid treatment promoted Nrf2 nuclear translocation, including colocalization with PML nuclear bodies.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6e22e6d8-74ad-5649-bdd3-cb677984f16f","mechanism_event_label":"The transcription factor moved toward the compartment where it can affect genes.","subject":{"id":"0b16e027-7a68-55ae-8e33-eae8b5cd1e9e","slug":"lycopene","display_name":"Lycopene","entity_type_key":"small_molecule"},"object":{"id":"cdca3452-2c25-5228-bfac-a71e0a9cda21","slug":"nrf2-nuclear-translocation","display_name":"NRF2 nuclear translocation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6e22e6d8-74ad-5649-bdd3-cb677984f16f","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-nrf2-location-event","event_type":"biochemical_relationship","label":"The transcription factor moved toward the compartment where it can affect genes.","description":"Carotenoid treatment promoted Nrf2 nuclear translocation, including colocalization with PML nuclear bodies.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dc472dd3-d383-567a-ab37-a9ec4e112df4","slug":"nfe2l2","display_name":"Human Nrf2 / NFE2L2","entity_type_key":"protein"},"role":"transcription_factor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0b16e027-7a68-55ae-8e33-eae8b5cd1e9e","slug":"lycopene","display_name":"Lycopene","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"cdca3452-2c25-5228-bfac-a71e0a9cda21","slug":"nrf2-nuclear-translocation","display_name":"NRF2 nuclear translocation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/lycopene-research/15657364.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a25b93d6074a1e560bf1e163aa70c511e99199b9bb62ccdffdbe70e51fcc7c32\", \"start_char\": 0, \"end_char\": 1890, \"text_sha256\": \"a25b93d6074a1e560bf1e163aa70c511e99199b9bb62ccdffdbe70e51fcc7c32\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Reporter, expression and dominant-negative transcription-factor experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Lycopene and ethanolic derivative extract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Unidentified derivative extract was active; direct binding of intact lycopene to KEAP1 was not established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lycopene research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lycopene","display_name":"Lycopene","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human cancer cell cultures","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The transcription factor moved toward the compartment where it can affect genes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lycopene-p15657364] Carotenoids activate the antioxidant response element transcription system. (2005). https://pubmed.ncbi.nlm.nih.gov/15657364/ DOI: 10.1158/1535-7163.177.4.1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"ARE transcription and phase II enzyme expression","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"11536d73-8fd5-5866-bbb1-cd38aece7fd1","evidence_kind":"source_excerpt","locator":"Lines 455-466","start_line":455,"end_line":466,"excerpt":"### lycopene-nrf2-location\nCarotenoid treatment promoted Nrf2 nuclear translocation, including colocalization with PML nuclear bodies.\nCondition category: normal\nnutrient_topic: Lycopene research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The transcription factor moved toward the compartment where it can affect genes.\norganism: Human cancer cell cultures\ntissue_or_cell_type: ARE transcription and phase II enzyme expression\nexperimental_model: Reporter, expression and dominant-negative transcription-factor experiments\nlimitations: Unidentified derivative extract was active; direct binding of intact lycopene to KEAP1 was not established.\nexposure: Lycopene and ethanolic derivative extract\nevidence_span: {\"source_cache\": \"artifacts/lycopene-research/15657364.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a25b93d6074a1e560bf1e163aa70c511e99199b9bb62ccdffdbe70e51fcc7c32\", \"start_char\": 0, \"end_char\": 1890, \"text_sha256\": \"a25b93d6074a1e560bf1e163aa70c511e99199b9bb62ccdffdbe70e51fcc7c32\"}\n[lycopene-p15657364] Carotenoids activate the antioxidant response element transcription system. (2005). https://pubmed.ncbi.nlm.nih.gov/15657364/ DOI: 10.1158/1535-7163.177.4.1","model_system":"Reporter, expression and dominant-negative transcription-factor experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [lycopene-p15657364] Carotenoids activate the antioxidant response element transcription system. (2005). https://pubmed.ncbi.nlm.nih.gov/15657364/ DOI: 10.1158/1535-7163.177.4.1","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d1e09874-8bde-548f-9664-26ad5eedb3c7","stable_key":"import-aad5a443-96e9-52eb-963a-812e90f9f896","title":"Lycopene: absorption, metabolism, nutrient connections and human outcomes (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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