{"id":"b6077fcc-e1ae-5a95-890a-dc4056baddd5","stable_key":"8b3cf083-ea9c-54f5-b42b-bf1fef5b2978:metformin-metformin-intestinal-thiamine","predicate":"reduces","statement":"Acute administration of metformin to wild-type mice reduced intestinal accumulation of thiamine.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"994149d7-fc28-549b-89b2-b519d4afdd4a","mechanism_event_label":"In a living animal, a dose of the drug lowered how much vitamin B1 the gut held.","subject":{"id":"519971b1-5aef-5ad7-8022-840144855cd0","slug":"metformin","display_name":"Metformin","entity_type_key":"drug"},"object":{"id":"6460a94a-5878-5e5d-bb34-3301c69910c3","slug":"mouse-intestinal-thiamine-accumulation","display_name":"Mouse intestinal thiamine accumulation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"994149d7-fc28-549b-89b2-b519d4afdd4a","stable_key":"8b3cf083-ea9c-54f5-b42b-bf1fef5b2978:metformin-metformin-intestinal-thiamine-event","event_type":"biochemical_relationship","label":"In a living animal, a dose of the drug lowered how much vitamin B1 the gut held.","description":"Acute administration of metformin to wild-type mice reduced intestinal accumulation of thiamine.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"role":"displaced_nutrient","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"519971b1-5aef-5ad7-8022-840144855cd0","slug":"metformin","display_name":"Metformin","entity_type_key":"drug"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6460a94a-5878-5e5d-bb34-3301c69910c3","slug":"mouse-intestinal-thiamine-accumulation","display_name":"Mouse intestinal thiamine accumulation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/metformin-research/24961373.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"77be4a228b3d067ca1e7ba44e8148f5fbda7956a23b42fb8281e9faf451a0e70\", \"start_char\": 0, \"end_char\": 1270, \"text_sha256\": \"77be4a228b3d067ca1e7ba44e8148f5fbda7956a23b42fb8281e9faf451a0e70\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Oct1-knockout and human OCT1 transgenic mice, metabolomics and isotopic uptake","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Metformin and phenformin against thiamine uptake; acute metformin in wild-type mice","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Identifies thiamine as an endogenous OCT1 substrate and shows competitive inhibition by metformin. The steatosis phenotype is a mouse phenotype.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Metformin research collection; topical membership is not evidence of a direct clinical effect, and pharmacological exposure is not dietary intake.","comparator":null,"unit":null,"notes":"","entity":{"slug":"metformin","display_name":"Metformin","entity_type_key":"drug"}},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"In a living animal, a dose of the drug lowered how much vitamin B1 the gut held.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[metformin-p24961373] OCT1 is a high-capacity thiamine transporter that regulates hepatic steatosis and is a target of metformin. (2014). https://pubmed.ncbi.nlm.nih.gov/24961373/ DOI: 10.1073/pnas.1314939111","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Liver and intestine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"f968c2cb-c8a3-5899-8b19-79ccdf9a04fd","evidence_kind":"source_excerpt","locator":"Lines 1256-1267","start_line":1256,"end_line":1267,"excerpt":"### metformin-metformin-intestinal-thiamine\nAcute administration of metformin to wild-type mice reduced intestinal accumulation of thiamine.\nCondition category: nutrient_deficiency\nnutrient_topic: Metformin research collection; topical membership is not evidence of a direct clinical effect, and pharmacological exposure is not dietary intake.\nplain_language: In a living animal, a dose of the drug lowered how much vitamin B1 the gut held.\norganism: Mouse\ntissue_or_cell_type: Liver and intestine\nexperimental_model: Oct1-knockout and human OCT1 transgenic mice, metabolomics and isotopic uptake\nlimitations: Identifies thiamine as an endogenous OCT1 substrate and shows competitive inhibition by metformin. The steatosis phenotype is a mouse phenotype.\nexposure: Metformin and phenformin against thiamine uptake; acute metformin in wild-type mice\nevidence_span: {\"source_cache\": \"artifacts/metformin-research/24961373.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"77be4a228b3d067ca1e7ba44e8148f5fbda7956a23b42fb8281e9faf451a0e70\", \"start_char\": 0, \"end_char\": 1270, \"text_sha256\": \"77be4a228b3d067ca1e7ba44e8148f5fbda7956a23b42fb8281e9faf451a0e70\"}\n[metformin-p24961373] OCT1 is a high-capacity thiamine transporter that regulates hepatic steatosis and is a target of metformin. (2014). https://pubmed.ncbi.nlm.nih.gov/24961373/ DOI: 10.1073/pnas.1314939111","model_system":"Oct1-knockout and human OCT1 transgenic mice, metabolomics and isotopic uptake","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [metformin-p24961373] OCT1 is a high-capacity thiamine transporter that regulates hepatic steatosis and is a target of metformin. (2014). https://pubmed.ncbi.nlm.nih.gov/24961373/ DOI: 10.1073/pnas.1314939111","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d0474bd9-7cfd-5695-a152-12f3633a6813","stable_key":"import-8b3cf083-ea9c-54f5-b42b-bf1fef5b2978","title":"Metformin: transport, molecular targets, gut mechanisms and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"0b51d0759aeeb88ac5b3a6277245a196b2bb024d7c32ec89379ed428fb06bfb5","revision_id":"0405c75e-f06e-5af7-a642-1777802f6004","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}