{"id":"b5a6b4bb-3c7f-58ed-8e99-9de70113d0e5","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-dhrs3-retinal-reduction","predicate":"converts","statement":"RDH10-activated DHRS3 reduced all-trans-retinal to retinol preferentially using NADPH.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"91113341-6f5f-590b-857c-392d2691c689","mechanism_event_label":"This reverse step restrains retinal available for acid production.","subject":{"id":"98441da8-56db-598c-9483-00e9c712656c","slug":"dhrs3","display_name":"Dehydrogenase/reductase 3 / DHRS3","entity_type_key":"protein"},"object":{"id":"148c57d8-c658-5769-89e8-1194cef2a782","slug":"retinol","display_name":"All-trans-retinol","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"91113341-6f5f-590b-857c-392d2691c689","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-dhrs3-retinal-reduction-event","event_type":"biochemical_relationship","label":"This reverse step restrains retinal available for acid production.","description":"RDH10-activated DHRS3 reduced all-trans-retinal to retinol preferentially using NADPH.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"89b4b04a-7637-56f4-ac55-501ad31d1f61","slug":"rdh10","display_name":"Retinol dehydrogenase 10 / RDH10","entity_type_key":"protein"},"role":"activating-partner","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"194eccf2-c7a5-52a6-a0c6-1c1f6d0f3a3e","slug":"all-trans-retinal","display_name":"All-trans-retinal","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4aba2a5e-8d06-5304-bb01-c0402b225a94","slug":"nadph","display_name":"NADPH","entity_type_key":"small_molecule"},"role":"reducing-cofactor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"be6e4670-953f-5a4e-97da-1e536a54e0e4","slug":"nadp-plus","display_name":"NADP+","entity_type_key":"small_molecule"},"role":"oxidized-cofactor-product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"98441da8-56db-598c-9483-00e9c712656c","slug":"dhrs3","display_name":"Dehydrogenase/reductase 3 / DHRS3","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"148c57d8-c658-5769-89e8-1194cef2a782","slug":"retinol","display_name":"All-trans-retinol","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"NADPH supports a retinoid buffering reaction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Figure 5 and Table 1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human RDH10/DHRS3 coexpression in HEK293 and Sf9 cells, plus Dhrs3-null embryos.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"DHRS3 coexpressed with RDH10; retinal plus NADPH.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cofactor dependency is not a dietary niacin intervention.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens proteins in Sf9 and HEK293 systems","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"outcome","value_text":"RDH10-activated DHRS3 reduced all-trans-retinal to retinol preferentially using NADPH.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This reverse step restrains retinal available for acid production.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[va-adams-2014] The retinaldehyde reductase activity of DHRS3 is reciprocally activated by retinol dehydrogenase 10 to control retinoid homeostasis (2014). https://pmc.ncbi.nlm.nih.gov/articles/PMC4031538/ DOI: 10.1074/jbc.M114.552257","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Microsomal/cellular retinoid system","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5d8674e4-564d-5e4a-b94b-892074b20397","evidence_kind":"source_excerpt","locator":"Lines 516-529","start_line":516,"end_line":529,"excerpt":"### va-dhrs3-retinal-reduction\nRDH10-activated DHRS3 reduced all-trans-retinal to retinol preferentially using NADPH.\nCondition category: normal\nnutrient_topic: Vitamin A research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This reverse step restrains retinal available for acid production.\norganism: Homo sapiens proteins in Sf9 and HEK293 systems\ntissue_or_cell_type: Microsomal/cellular retinoid system\nexperimental_model: Human RDH10/DHRS3 coexpression in HEK293 and Sf9 cells, plus Dhrs3-null embryos.\nlimitations: Cofactor dependency is not a dietary niacin intervention.\nexposure: DHRS3 coexpressed with RDH10; retinal plus NADPH.\noutcome: RDH10-activated DHRS3 reduced all-trans-retinal to retinol preferentially using NADPH.\nevidence_location: Figure 5 and Table 1\ncross_nutrient: NADPH supports a retinoid buffering reaction.\n[va-adams-2014] The retinaldehyde reductase activity of DHRS3 is reciprocally activated by retinol dehydrogenase 10 to control retinoid homeostasis (2014). https://pmc.ncbi.nlm.nih.gov/articles/PMC4031538/ DOI: 10.1074/jbc.M114.552257","model_system":"Human RDH10/DHRS3 coexpression in HEK293 and Sf9 cells, plus Dhrs3-null embryos.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [va-adams-2014] The retinaldehyde reductase activity of DHRS3 is reciprocally activated by retinol dehydrogenase 10 to control retinoid homeostasis (2014). https://pmc.ncbi.nlm.nih.gov/articles/PMC4031538/ DOI: 10.1074/jbc.M114.552257","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0c5474e2-be48-547b-b287-9c07a59c9ff1","stable_key":"import-dff9f743-3766-5f20-a71e-d6d2bd5bfb6f","title":"Vitamin A: forms, mechanisms, deficiency and excess (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b217224b262abbd77d6a0c8c9beefff9c022fae4d31e2a5273915d769b9a6546","revision_id":"61008909-871f-575c-b5e2-21542689ea3c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}