{"id":"b58bef4f-b232-5e82-8d7c-2d7441c47b52","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-u266-ampk-inhibitor","predicate":"blocks","statement":"Compound C blocked fisetin-induced apoptosis in human U266 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"33debd88-55e5-598b-98b9-17eaaa7326cd","mechanism_event_label":"A kinase-pathway inhibitor also weakened the response.","subject":{"id":"9dc62af6-83b4-5398-84ad-1a2de0b82f5f","slug":"compound-c","display_name":"Compound C / dorsomorphin","entity_type_key":"small_molecule"},"object":{"id":"07297625-7ece-5fd3-aa02-66b83d0c7bae","slug":"human-u266-fisetin-apoptosis","display_name":"Fisetin-associated apoptosis in human U266 myeloma cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"33debd88-55e5-598b-98b9-17eaaa7326cd","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-u266-ampk-inhibitor-event","event_type":"observed_relationship","label":"A kinase-pathway inhibitor also weakened the response.","description":"Compound C blocked fisetin-induced apoptosis in human U266 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9dc62af6-83b4-5398-84ad-1a2de0b82f5f","slug":"compound-c","display_name":"Compound C / dorsomorphin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"07297625-7ece-5fd3-aa02-66b83d0c7bae","slug":"human-u266-fisetin-apoptosis","display_name":"Fisetin-associated apoptosis in human U266 myeloma cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"95bc303f-ad04-59ea-8e1e-32f9e8b72c56","slug":"human-ampk-complexes","display_name":"Human AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Pharmacological AMPK-pathway perturbation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Compound C has off-target activity; this is not equivalent to AMPK-specific genetic proof.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A kinase-pathway inhibitor also weakened the response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Activation of reactive oxygen species/AMP activated protein kinase signaling mediates fisetin-induced apoptosis in multiple myeloma U266 cells. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22261340/ · DOI 10.1016/j.canlet.2012.01.008","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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