{"id":"b55fac4c-b2e4-53b5-814e-e9098fd9253e","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-metabolite-secretion","predicate":"impairs","statement":"Sulfanilic acid pretreatment impaired CCK-8-evoked trypsin secretion from AR42J cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"10b8c29a-b5ee-5dd0-a35e-f1e4d2e75b47","mechanism_event_label":"Sulfanilic acid pretreatment impaired CCK-8-evoked trypsin secretion from AR42J cells.","subject":{"id":"f0f532e0-d3c7-5f4f-a9de-a979cd031bae","slug":"sulfanilic-acid","display_name":"Sulfanilic acid","entity_type_key":"small_molecule"},"object":{"id":"bd4ced0d-97de-5388-bfe3-e64b788a2f84","slug":"rat-ar42j-cck-stimulated-trypsin-secretion","display_name":"CCK-8-stimulated trypsin secretion by rat AR42J cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"10b8c29a-b5ee-5dd0-a35e-f1e4d2e75b47","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-metabolite-secretion-event","event_type":"observed_relationship","label":"Sulfanilic acid pretreatment impaired CCK-8-evoked trypsin secretion from AR42J cells.","description":"Sulfanilic acid pretreatment impaired CCK-8-evoked trypsin secretion from AR42J cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f0f532e0-d3c7-5f4f-a9de-a979cd031bae","slug":"sulfanilic-acid","display_name":"Sulfanilic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bd4ced0d-97de-5388-bfe3-e64b788a2f84","slug":"rat-ar42j-cck-stimulated-trypsin-secretion","display_name":"CCK-8-stimulated trypsin secretion by rat AR42J cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"3ced4333-305c-5dad-b64f-2b3585323bea","slug":"cck-8","display_name":"Cholecystokinin octapeptide / CCK-8","entity_type_key":"peptide"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Sulfanilic acid 1 micromolar-1 mM; mitochondrial depolarization reported at 1 mM","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute calcium time course; other assay intervals not specified in abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract; unrecovered method details explicitly retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific experimental curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat pancreatic AR42J cell line","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The metabolite was administered directly. No measured oral tartrazine-to-pancreas exposure or pancreatitis outcome is established. Oxidant production was reported to be calcium-independent.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tartrazine food-colorant chapter; nutrient, drug and peptide interactions retain their models and limits.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rat pancreatic AR42J cell line","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Sulfanilic acid pretreatment impaired CCK-8-evoked trypsin secretion from AR42J cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Sulfanilic acid increases intracellular free-calcium concentration, induces reactive oxygen species production and impairs trypsin secretion in pancreatic AR42J cells. (2018). https://pubmed.ncbi.nlm.nih.gov/29986830/ DOI: 10.1016/j.fct.2018.07.001","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro metabolite exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Calcium, redox, mitochondrial and secretory assays","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cdb65a04-91f9-568a-94ef-4b8cd271f76c","evidence_kind":"source_excerpt","locator":"Lines 193-202","start_line":193,"end_line":202,"excerpt":"## tartrazine-metabolite-secretion\nSulfanilic acid pretreatment impaired CCK-8-evoked trypsin secretion from AR42J cells.\nModel/species: Rat pancreatic AR42J cell line\nTissue: Calcium, redox, mitochondrial and secretory assays\nExposure: Sulfanilic acid 1 micromolar-1 mM; mitochondrial depolarization reported at 1 mM\nRoute: In vitro metabolite exposure\nDuration: Acute calcium time course; other assay intervals not specified in abstract\nLimits: The metabolite was administered directly. No measured oral tartrazine-to-pancreas exposure or pancreatitis outcome is established. Oxidant production was reported to be calcium-independent.\nPrimary reference: Sulfanilic acid increases intracellular free-calcium concentration, induces reactive oxygen species production and impairs trypsin secretion in pancreatic AR42J cells. (2018). https://pubmed.ncbi.nlm.nih.gov/29986830/ DOI: 10.1016/j.fct.2018.07.001\nAccess: Primary PubMed abstract; unrecovered method details explicitly retained.","model_system":"Rat pancreatic AR42J cell line","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; primary citation retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d9540941-68b4-5a0d-a5c1-828665a918e2","stable_key":"import-792249b7-a38d-5f43-afb6-2936818a3fa2","title":"Tartrazine: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eighteen primary studies. Study-specific citations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"b83a0113a4b743adc91c24504e488f47e10665386b299c4cb0543da8948f4246","revision_id":"3769070e-6af4-5b02-9d8e-f23c84edba1b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}