{"id":"b55bffc1-f801-5159-b60d-fb277db980f4","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-dnak-binding","predicate":"allosterically_binds","statement":"NMR located myricetin binding between DnaK subdomains IB/IIB; binding impaired interaction with DnaJ.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0682b714-bf54-531b-b9ce-e3defc4b6fd4","mechanism_event_label":"A compound can inhibit a protein partnership rather than the catalytic site.","subject":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"object":{"id":"3ef06b48-8daf-5aba-8f16-d43fb24e560a","slug":"ecoli-dnak","display_name":"Escherichia coli DnaK chaperone","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"0682b714-bf54-531b-b9ce-e3defc4b6fd4","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-dnak-binding-event","event_type":"observed_relationship","label":"A compound can inhibit a protein partnership rather than the catalytic site.","description":"NMR located myricetin binding between DnaK subdomains IB/IIB; binding impaired interaction with DnaJ.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3ef06b48-8daf-5aba-8f16-d43fb24e560a","slug":"ecoli-dnak","display_name":"Escherichia coli DnaK chaperone","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3e8c3c69-f973-51f7-bdfe-15fb3b1459d9","slug":"ecoli-dnaj","display_name":"Escherichia coli DnaJ co-chaperone","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Reconstituted E. coli chaperones and NMR.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not generalize directly to human HSP70.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Myricetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A compound can inhibit a protein partnership rather than the catalytic site.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Chemical screens against a reconstituted multiprotein complex: myricetin blocks DnaJ regulation of DnaK through an allosteric mechanism. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21338918/ · DOI 10.1016/j.chembiol.2010.12.010","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0636a60c-cdad-5d6f-9e03-d399dba6342a","evidence_kind":"source_excerpt","locator":"Lines 580-586","start_line":580,"end_line":586,"excerpt":"## myricetin-dnak-binding\nA compound can inhibit a protein partnership rather than the catalytic site.\nNMR located myricetin binding between DnaK subdomains IB/IIB; binding impaired interaction with DnaJ.\nModel: Reconstituted E. coli chaperones and NMR.\nLimitations: Do not generalize directly to human HSP70.\nEvidence access: Primary full text\nChemical screens against a reconstituted multiprotein complex: myricetin blocks DnaJ regulation of DnaK through an allosteric mechanism. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21338918/ · DOI 10.1016/j.chembiol.2010.12.010","model_system":"Reconstituted E. coli chaperones and NMR.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"58045cd9-3b27-5469-bb4b-e1c6a9bdca34","stable_key":"import-8182578e-8d21-598e-a3cb-e62d480d2251","title":"Myricetin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"0abf512275cf3aae9e4ec8544f1cde97e0155d0cfb5be12a193cdd5a898386a7","revision_id":"422e342f-a55c-5e73-ba98-43df560f49b9","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}