{"id":"b545b285-b1c1-5e08-85ca-25fc442f1bb8","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-myoblast-slc4a11-dependence","predicate":"required_for_response_of","statement":"Slc4a11 silencing abolished the boron-associated enhancement of adhesion and tension in C2C12 cells; replacing fibronectin with laminin-111 also abolished the response.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3f9043bf-da4f-5b83-bf86-86a9dd1a8cc2","mechanism_event_label":"Both the protein and the cells’ attachment surface mattered.","subject":{"id":"d4cb804f-b7de-5d1b-8dbe-085d58fa29a6","slug":"mouse-slc4a11","display_name":"Mouse Slc4a11 / NaBC1","entity_type_key":"protein"},"object":{"id":"6ab563a7-ba40-512e-82cf-548db9cc91d2","slug":"cellular-adhesion-tension","display_name":"Cell adhesion and intracellular mechanical tension","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3f9043bf-da4f-5b83-bf86-86a9dd1a8cc2","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-myoblast-slc4a11-dependence-event","event_type":"biochemical_relationship","label":"Both the protein and the cells’ attachment surface mattered.","description":"Slc4a11 silencing abolished the boron-associated enhancement of adhesion and tension in C2C12 cells; replacing fibronectin with laminin-111 also abolished the response.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"cfbf4db0-5a59-56fe-a2b8-2176eac27237","slug":"borax","display_name":"Sodium tetraborate / borax","entity_type_key":"chemical_species"},"role":"exposure","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d4cb804f-b7de-5d1b-8dbe-085d58fa29a6","slug":"mouse-slc4a11","display_name":"Mouse Slc4a11 / NaBC1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6ab563a7-ba40-512e-82cf-548db9cc91d2","slug":"cellular-adhesion-tension","display_name":"Cell adhesion and intracellular mechanical tension","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/boron-research/40270477.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e55fb244b3c6f4adc9c4b1ab4ed2e8bb2a866b01bc9b50b16ed85e1f4737aeab\", \"start_char\": 0, \"end_char\": 997, \"text_sha256\": \"e55fb244b3c6f4adc9c4b1ab4ed2e8bb2a866b01bc9b50b16ed85e1f4737aeab\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Substrate-stiffness experiments, traction measurements and Slc4a11 silencing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Soluble boron supplied as borax; reported experimental concentrations 0.59 and 1.47 mM; variable substrate stiffness","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Protein-dependence of a cell-mechanics phenotype does not demonstrate transmembrane borate transport. The authors use the historical transporter label; human transport remains disputed. Matrix coating and exposure are essential context.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Boron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"boron","display_name":"Boron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse C2C12 myoblasts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Both the protein and the cells’ attachment surface mattered.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[boron-p40270477] NaBC1 Boron Transporter Enables Myoblast Response to Substrate Rigidity via Fibronectin-Binding Integrins. (2025). https://pubmed.ncbi.nlm.nih.gov/40270477/ DOI: 10.1002/advs.202407548","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cells on fibronectin-functionalized polyacrylamide hydrogels","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"32b26936-3e60-5013-9755-3dd3e9696d10","evidence_kind":"source_excerpt","locator":"Lines 586-597","start_line":586,"end_line":597,"excerpt":"### boron-myoblast-slc4a11-dependence\nSlc4a11 silencing abolished the boron-associated enhancement of adhesion and tension in C2C12 cells; replacing fibronectin with laminin-111 also abolished the response.\nCondition category: machinery_impairment\nnutrient_topic: Boron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Both the protein and the cells’ attachment surface mattered.\norganism: Mouse C2C12 myoblasts\ntissue_or_cell_type: Cells on fibronectin-functionalized polyacrylamide hydrogels\nexperimental_model: Substrate-stiffness experiments, traction measurements and Slc4a11 silencing\nlimitations: Protein-dependence of a cell-mechanics phenotype does not demonstrate transmembrane borate transport. The authors use the historical transporter label; human transport remains disputed. Matrix coating and exposure are essential context.\nexposure: Soluble boron supplied as borax; reported experimental concentrations 0.59 and 1.47 mM; variable substrate stiffness\nevidence_span: {\"source_cache\": \"artifacts/boron-research/40270477.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e55fb244b3c6f4adc9c4b1ab4ed2e8bb2a866b01bc9b50b16ed85e1f4737aeab\", \"start_char\": 0, \"end_char\": 997, \"text_sha256\": \"e55fb244b3c6f4adc9c4b1ab4ed2e8bb2a866b01bc9b50b16ed85e1f4737aeab\"}\n[boron-p40270477] NaBC1 Boron Transporter Enables Myoblast Response to Substrate Rigidity via Fibronectin-Binding Integrins. 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