{"id":"b5250499-ac21-54e2-9058-c41e1cb77dfd","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-nfkb","predicate":"activates","statement":"Adding t10,c12 CLA promoted NF-kappa-B activation, including p65 phosphorylation and nuclear translocation with I-kappa-B-alpha degradation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a12df437-f48e-5282-b9ad-fc1f1c6a8f77","mechanism_event_label":"Inflammatory signaling became active in the fat-cell cultures.","subject":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"object":{"id":"ecf9653e-8710-540d-953a-78ef96bf4752","slug":"rela","display_name":"Human NF-kappa-B p65 / RELA","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"a12df437-f48e-5282-b9ad-fc1f1c6a8f77","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-nfkb-event","event_type":"observed_relationship","label":"Inflammatory signaling became active in the fat-cell cultures.","description":"Adding t10,c12 CLA promoted NF-kappa-B activation, including p65 phosphorylation and nuclear translocation with I-kappa-B-alpha degradation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ecf9653e-8710-540d-953a-78ef96bf4752","slug":"rela","display_name":"Human NF-kappa-B p65 / RELA","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4b7e6069-53d4-58cf-be3c-d63e362025bf","slug":"nfkbia","display_name":"Human I-kappa-B alpha / NFKBIA","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human adipocyte/stromal-vascular cultures and explants.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Different cell populations contribute to the response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Inflammatory signaling became active in the fat-cell cultures.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Conjugated linoleic acid promotes human adipocyte insulin resistance through NFkappaB-dependent cytokine production. · 2005 · https://pubmed.ncbi.nlm.nih.gov/16155293/ · DOI 10.1074/jbc.M508159200","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f1bb091c-4c78-5372-9694-8e3f03e67e86","evidence_kind":"source_excerpt","locator":"Lines 126-132","start_line":126,"end_line":132,"excerpt":"## cla-nfkb\nInflammatory signaling became active in the fat-cell cultures.\nAdding t10,c12 CLA promoted NF-kappa-B activation, including p65 phosphorylation and nuclear translocation with I-kappa-B-alpha degradation.\nModel: Human adipocyte/stromal-vascular cultures and explants.\nLimitations: Different cell populations contribute to the response.\nEvidence access: Primary abstract\nConjugated linoleic acid promotes human adipocyte insulin resistance through NFkappaB-dependent cytokine production. · 2005 · https://pubmed.ncbi.nlm.nih.gov/16155293/ · DOI 10.1074/jbc.M508159200","model_system":"Human adipocyte/stromal-vascular cultures and explants.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"45cfa6e3-2e7d-57c1-8c35-65302b7b71db","stable_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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