{"id":"b52440ca-77ec-55b9-8b52-dda842307394","stable_key":"708c252b-0aef-5cec-95bf-99d19578214c:sfx01-bb3rc61-pstat3","predicate":"has_contextual_experimental_result","statement":"SFX-01 reduced phosphorylated STAT3 relative to antiestrogen-only treatments in BB3RC61 cells.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"683c9970-e667-5f53-8eda-6c63e07d580a","mechanism_event_label":"sfx01-bb3rc61-pstat3","subject":{"id":"80b20bc7-d9e7-5909-9e77-838b0e3c8486","slug":"sfx-01","display_name":"SFX-01, sulforaphane stabilized in an alpha-cyclodextrin complex","entity_type_key":"chemical_species"},"object":{"id":"7f3bce97-3272-5acb-bbc2-fb7830e30278","slug":"human-whole-cell-pstat3-abundance","display_name":"Whole-cell phosphorylated STAT3 abundance in human cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"683c9970-e667-5f53-8eda-6c63e07d580a","stable_key":"708c252b-0aef-5cec-95bf-99d19578214c:sfx01-bb3rc61-pstat3","event_type":"experimental_result","label":"sfx01-bb3rc61-pstat3","description":"SFX-01 reduced phosphorylated STAT3 relative to antiestrogen-only treatments in BB3RC61 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80b20bc7-d9e7-5909-9e77-838b0e3c8486","slug":"sfx-01","display_name":"SFX-01, sulforaphane stabilized in an alpha-cyclodextrin complex","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7f3bce97-3272-5acb-bbc2-fb7830e30278","slug":"human-whole-cell-pstat3-abundance","display_name":"Whole-cell phosphorylated STAT3 abundance in human cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dc129d3d-aaa0-5511-9a60-3e3880b0eb0f","slug":"stat3","display_name":"STAT3","entity_type_key":"protein"},"role":"measured protein","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"role":"compound in stabilized formulation","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human antiestrogen-resistant metastatic breast cancer sample BB3RC61","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"72-hour treatment. Supplementary reagent protocol uses 5 micromolar SFX-01, 1 micromolar 4-OH-tamoxifen or 0.1 micromolar fulvestrant; these concentrations are explicitly stated for cell lines, and patient-specific dosing is not independently confirmed here.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The chemical probe and stabilized formulation are separate experimental actors. Capture supports engagement but does not identify the modified STAT3 cysteine or demonstrate that APT2 mediates the effect. No GPX4 time-course endpoint. Primary cell/PDX studies are not evidence of benefit from dietary broccoli.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"Figure 4d; Results: SFX-01 targets STAT3 signaling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41388-020-1335-z","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5098f577-456f-5224-a86b-72cbfd1cbc67","evidence_kind":"source_excerpt","locator":"Lines 96-102","start_line":96,"end_line":102,"excerpt":"SFX-01 reduced phosphorylated STAT3 relative to antiestrogen-only treatments in BB3RC61 cells.\nprimary_references: https://doi.org/10.1038/s41388-020-1335-z\nprimary_locator: Figure 4d; Results: SFX-01 targets STAT3 signaling.\norganism: Human\nexperimental_model: Human antiestrogen-resistant metastatic breast cancer sample BB3RC61\nexposure: 72-hour treatment. Supplementary reagent protocol uses 5 micromolar SFX-01, 1 micromolar 4-OH-tamoxifen or 0.1 micromolar fulvestrant; these concentrations are explicitly stated for cell lines, and patient-specific dosing is not independently confirmed here.\nlimitations: The chemical probe and stabilized formulation are separate experimental actors. Capture supports engagement but does not identify the modified STAT3 cysteine or demonstrate that APT2 mediates the effect. No GPX4 time-course endpoint. Primary cell/PDX studies are not evidence of benefit from dietary broccoli.","model_system":"Human antiestrogen-resistant metastatic breast cancer sample BB3RC61","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curator paraphrase of the specified primary result; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01fc674f-07c6-58fd-b1e7-877478a4472e","stable_key":"import-708c252b-0aef-5cec-95bf-99d19578214c","title":"APT2-STAT3 cycling and a competing sulforaphane target route","document_type":"imported_text","citation_label":"Selected primary observations: 10.1038/s41586-020-2799-2 and 10.1038/s41388-020-1335-z.","file_path":"","sha256":"b1b245468dac7acc94a2fef690d994124b2bc4fc5f92135173cf5636af391395","revision_id":"d84b8419-69dd-50d9-b587-5627ed414591","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}