{"id":"b49f46a2-b9a2-5826-8da3-f1c68f355eae","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-myotube-glucose","predicate":"increases_tested","statement":"Isoleucine at 1-10 mM increased 2-deoxyglucose uptake in insulin-free C2C12 myotube assays.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8010b630-58b1-540c-b8b7-7e672ff0ee92","mechanism_event_label":"Muscle cells responded without added insulin in this experiment.","subject":{"id":"c85b327e-f132-5d9a-8e4b-c2976fcc0373","slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"},"object":{"id":"58eae8ee-51c7-599d-adc1-beedb24ca12e","slug":"mouse-c2c12-glucose-uptake","display_name":"Glucose uptake in mouse C2C12 myotubes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8010b630-58b1-540c-b8b7-7e672ff0ee92","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-myotube-glucose-event","event_type":"observed_relationship","label":"Muscle cells responded without added insulin in this experiment.","description":"Isoleucine at 1-10 mM increased 2-deoxyglucose uptake in insulin-free C2C12 myotube assays.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c85b327e-f132-5d9a-8e4b-c2976fcc0373","slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"58eae8ee-51c7-599d-adc1-beedb24ca12e","slug":"mouse-c2c12-glucose-uptake","display_name":"Glucose uptake in mouse C2C12 myotubes","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse C2C12 cells; millimolar amino-acid exposures.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Insulin-free culture is not proof of insulin independence in all intact organisms.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Isoleucine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Muscle cells responded without added insulin in this experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Isoleucine, a potent plasma glucose-lowering amino acid, stimulates glucose uptake in C2C12 myotubes. · 2003 · https://pubmed.ncbi.nlm.nih.gov/14651987/ · DOI 10.1016/j.bbrc.2003.11.039","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3be55cf4-d73f-51a3-9e90-07796de8eb77","evidence_kind":"source_excerpt","locator":"Lines 346-352","start_line":346,"end_line":352,"excerpt":"## isoleucine-myotube-glucose\nMuscle cells responded without added insulin in this experiment.\nIsoleucine at 1-10 mM increased 2-deoxyglucose uptake in insulin-free C2C12 myotube assays.\nModel: Mouse C2C12 cells; millimolar amino-acid exposures.\nLimitations: Insulin-free culture is not proof of insulin independence in all intact organisms.\nEvidence access: Primary abstract\nIsoleucine, a potent plasma glucose-lowering amino acid, stimulates glucose uptake in C2C12 myotubes. · 2003 · https://pubmed.ncbi.nlm.nih.gov/14651987/ · DOI 10.1016/j.bbrc.2003.11.039","model_system":"Mouse C2C12 cells; millimolar amino-acid exposures.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4b00f118-00fd-5cef-be97-13125eb958e9","stable_key":"import-09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2","title":"L-Isoleucine: transport, translation, catabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"2058ff0da379586994e7fda6ae0086c60fa3173542bfaf1fb3871ad3e09f9c6c","revision_id":"f32192d4-9913-5d35-acbc-cf90ff17995e","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}