{"id":"b47a83e6-ddf0-52f0-be42-ddc02beddc49","stable_key":"e13b03e1-a614-543a-ac1b-97df18cfe30d:iron-pcbp-depletion","predicate":"loss_increased","statement":"PCBP1 depletion inhibited ferritin iron loading and increased cytosolic iron pools in human cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"016466c3-225c-5f8e-8cd4-27b9f1109a31","mechanism_event_label":"Less iron in storage can coexist with more potentially reactive iron outside the store.","subject":{"id":"70d4dfb6-5f8c-5ed2-b3fd-5aa7e7b1ca7d","slug":"pcbp1","display_name":"Human poly(rC)-binding protein 1 / PCBP1","entity_type_key":"protein"},"object":{"id":"d1a76588-8d56-5ba9-9fd1-16a82dc52b94","slug":"labile-cellular-iron","display_name":"Cellular labile iron pool","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"016466c3-225c-5f8e-8cd4-27b9f1109a31","stable_key":"e13b03e1-a614-543a-ac1b-97df18cfe30d:iron-pcbp-depletion-event","event_type":"biochemical_relationship","label":"Less iron in storage can coexist with more potentially reactive iron outside the store.","description":"PCBP1 depletion inhibited ferritin iron loading and increased cytosolic iron pools in human cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5ee66abb-7d76-5b09-bd06-77d39d0e3bcd","slug":"mammalian-ferritin","display_name":"Mammalian ferritin protein complexes","entity_type_key":"protein_family"},"role":"underloaded_store","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"70d4dfb6-5f8c-5ed2-b3fd-5aa7e7b1ca7d","slug":"pcbp1","display_name":"Human poly(rC)-binding protein 1 / PCBP1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d1a76588-8d56-5ba9-9fd1-16a82dc52b94","slug":"labile-cellular-iron","display_name":"Cellular labile iron pool","entity_type_key":"chemical_species"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/iron-research/18511687.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"d8000e5c5f59dc72e9697d5aac77ce5070a1c92f6cada7e863088ec917684c68\", \"start_char\": 0, \"end_char\": 703, \"text_sha256\": \"d8000e5c5f59dc72e9697d5aac77ce5070a1c92f6cada7e863088ec917684c68\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Yeast expression, in-vitro binding/loading and human-cell depletion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"PCBP1 expression and depletion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Chaperone-mediated distribution is distinct from total cellular iron.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human PCBP1 and ferritin in yeast and human cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Less iron in storage can coexist with more potentially reactive iron outside the store.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[iron-p18511687] A cytosolic iron chaperone that delivers iron to ferritin. (2008). https://pubmed.ncbi.nlm.nih.gov/18511687/ DOI: 10.1126/science.1157643","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cytosol","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"5cb9301f-8972-594a-8e4a-45b471c70dc9","evidence_kind":"source_excerpt","locator":"Lines 602-613","start_line":602,"end_line":613,"excerpt":"### iron-pcbp-depletion\nPCBP1 depletion inhibited ferritin iron loading and increased cytosolic iron pools in human cells.\nCondition category: machinery_impairment\nnutrient_topic: Iron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Less iron in storage can coexist with more potentially reactive iron outside the store.\norganism: Human PCBP1 and ferritin in yeast and human cells\ntissue_or_cell_type: Cytosol\nexperimental_model: Yeast expression, in-vitro binding/loading and human-cell depletion\nlimitations: Chaperone-mediated distribution is distinct from total cellular iron.\nexposure: PCBP1 expression and depletion\nevidence_span: {\"source_cache\": \"artifacts/iron-research/18511687.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"d8000e5c5f59dc72e9697d5aac77ce5070a1c92f6cada7e863088ec917684c68\", \"start_char\": 0, \"end_char\": 703, \"text_sha256\": \"d8000e5c5f59dc72e9697d5aac77ce5070a1c92f6cada7e863088ec917684c68\"}\n[iron-p18511687] A cytosolic iron chaperone that delivers iron to ferritin. 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