{"id":"b465998e-5074-5791-af7c-4b18c2c068ff","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mitochondrial-potential-supports-mrs2-uptake","predicate":"supports","statement":"Dissipating membrane potential reduced Mg uptake in the human MRS2 mitochondrial assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"d206807e-dad2-5935-9e65-5c0f0e760cd6","mechanism_event_label":"The inner membrane electrical gradient helps drive magnesium into mitochondria.","subject":{"id":"2f9332e0-0138-5e42-8c7b-8f8f45615782","slug":"mitochondrial-membrane-potential","display_name":"Inner mitochondrial membrane potential","entity_type_key":"cellular_process"},"object":{"id":"b47df614-7498-5a76-85cf-b7ffbdb970d9","slug":"mitochondrial-magnesium-uptake","display_name":"Mitochondrial magnesium uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d206807e-dad2-5935-9e65-5c0f0e760cd6","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mitochondrial-potential-supports-mrs2-uptake-event","event_type":"biochemical_relationship","label":"The inner membrane electrical gradient helps drive magnesium into mitochondria.","description":"Dissipating membrane potential reduced Mg uptake in the human MRS2 mitochondrial assay.","status":"provisional","compartment":{"slug":"inner-mitochondrial-membrane","display_name":"Inner mitochondrial membrane"},"participants":[{"entity":{"id":"6adc56ba-63e3-5558-ade0-7c84612e1e98","slug":"mrs2","display_name":"Human mitochondrial MRS2","entity_type_key":"protein"},"role":"channel","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"transported-ion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2f9332e0-0138-5e42-8c7b-8f8f45615782","slug":"mitochondrial-membrane-potential","display_name":"Inner mitochondrial membrane potential","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b47df614-7498-5a76-85cf-b7ffbdb970d9","slug":"mitochondrial-magnesium-uptake","display_name":"Mitochondrial magnesium uptake","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence-system","value_text":"FCCP or valinomycin exposure of isolated mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"FCCP or valinomycin exposure of isolated mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pharmacological potential collapse can affect other mitochondrial properties; does not imply direct ATP hydrolysis by MRS2.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The inner membrane electrical gradient helps drive magnesium into mitochondria.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[human-mrs2-2023-permeation] Molecular basis of Mg2+ permeation through the human mitochondrial Mrs2 channel (2023). https://www.nature.com/articles/s41467-023-40516-2 DOI: 10.1038/s41467-023-40516-2","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"HEK293F-derived mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HEK293F-derived mitochondria","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e441341d-27d4-5a09-b840-762e78b72753","evidence_kind":"source_excerpt","locator":"Lines 1235-1246","start_line":1235,"end_line":1246,"excerpt":"### mitochondrial-potential-supports-mrs2-uptake\nDissipating membrane potential reduced Mg uptake in the human MRS2 mitochondrial assay.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The inner membrane electrical gradient helps drive magnesium into mitochondria.\norganism: Human\ntissue_or_cell_type: HEK293F-derived mitochondria\nexperimental_model: FCCP or valinomycin exposure of isolated mitochondria\nlimitations: Pharmacological potential collapse can affect other mitochondrial properties; does not imply direct ATP hydrolysis by MRS2.\nevidence-system: FCCP or valinomycin exposure of isolated mitochondria\ntissue: HEK293F-derived mitochondria\n[human-mrs2-2023-permeation] Molecular basis of Mg2+ permeation through the human mitochondrial Mrs2 channel (2023). https://www.nature.com/articles/s41467-023-40516-2 DOI: 10.1038/s41467-023-40516-2","model_system":"FCCP or valinomycin exposure of isolated mitochondria","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [human-mrs2-2023-permeation] Molecular basis of Mg2+ permeation through the human mitochondrial Mrs2 channel (2023). https://www.nature.com/articles/s41467-023-40516-2 DOI: 10.1038/s41467-023-40516-2","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}