{"id":"b44fdb91-a3ba-5315-b482-8fc9cf29e35f","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-restriction-creatine-profile","predicate":"restriction_lowered_plasma_creatine","statement":"Plasma creatine decreased 40% in both meal states; creatinine fell 9% and dimethylglycine 16% postprandially.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"3a1d02c8-4415-5ebf-ada0-65063eabaadb","mechanism_event_label":"B6 restriction changed selected methylation-related metabolites.","subject":{"id":"c0beb226-8842-501f-a147-cac0d9fa7060","slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"},"object":{"id":"9f5df558-ed3d-5cda-b5ca-0f90f7aa6a8c","slug":"creatine","display_name":"Creatine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"3a1d02c8-4415-5ebf-ada0-65063eabaadb","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-restriction-creatine-profile-event","event_type":"observed_intervention","label":"B6 restriction changed selected methylation-related metabolites.","description":"Plasma creatine decreased 40% in both meal states; creatinine fell 9% and dimethylglycine 16% postprandially.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9c25f59f-e020-5dbb-b39b-90ad59525d84","slug":"creatinine","display_name":"Creatinine","entity_type_key":"small_molecule"},"role":"measured_metabolite","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7b776af6-a5ce-5b2d-8d29-7ded4362a56c","slug":"dimethylglycine","display_name":"Dimethylglycine","entity_type_key":"small_molecule"},"role":"measured_metabolite","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c0beb226-8842-501f-a147-cac0d9fa7060","slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9f5df558-ed3d-5cda-b5ca-0f90f7aa6a8c","slug":"creatine","display_name":"Creatine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Plasma metabolites connect B6 status with one-carbon metabolism without establishing a complete causal chain.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Twenty-three healthy adults; paired pre/postprandial LC-MS/MS metabolite profiling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"28 days below 0.35 mg/day B6. Related restriction research program; do not count paper totals as independent trial replications.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This does not establish exhausted SAM, impaired muscle phosphocreatine or a direct PLP requirement for creatine methylation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"B6 restriction changed selected methylation-related metabolites.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b6-dasilva2013] Metabolite profile analysis reveals functional effects of 28-day vitamin B-6 restriction on one-carbon metabolism and tryptophan catabolic pathways in healthy men and women (2013). https://pubmed.ncbi.nlm.nih.gov/23966327/ DOI: 10.3945/jn.113.180588","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Human blood and whole-body measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"084cf202-8ac2-5925-a286-ea5ceddd69ab","evidence_kind":"source_excerpt","locator":"Lines 1423-1434","start_line":1423,"end_line":1434,"excerpt":"### b6-restriction-creatine-profile\nPlasma creatine decreased 40% in both meal states; creatinine fell 9% and dimethylglycine 16% postprandially.\nCondition category: nutrient_deficiency\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: B6 restriction changed selected methylation-related metabolites.\norganism: Homo sapiens\ntissue_or_cell_type: Human blood and whole-body measurements\nexperimental_model: Twenty-three healthy adults; paired pre/postprandial LC-MS/MS metabolite profiling.\nlimitations: This does not establish exhausted SAM, impaired muscle phosphocreatine or a direct PLP requirement for creatine methylation.\nexposure: 28 days below 0.35 mg/day B6. Related restriction research program; do not count paper totals as independent trial replications.\ncross_nutrient: Plasma metabolites connect B6 status with one-carbon metabolism without establishing a complete causal chain.\n[b6-dasilva2013] Metabolite profile analysis reveals functional effects of 28-day vitamin B-6 restriction on one-carbon metabolism and tryptophan catabolic pathways in healthy men and women (2013). https://pubmed.ncbi.nlm.nih.gov/23966327/ DOI: 10.3945/jn.113.180588","model_system":"Twenty-three healthy adults; paired pre/postprandial LC-MS/MS metabolite profiling.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b6-dasilva2013] Metabolite profile analysis reveals functional effects of 28-day vitamin B-6 restriction on one-carbon metabolism and tryptophan catabolic pathways in healthy men and women (2013). https://pubmed.ncbi.nlm.nih.gov/23966327/ DOI: 10.3945/jn.113.180588","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}