{"id":"b4433e88-0796-5b9e-845f-6392485e8c9e","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-hepatocyte-gsta1-null","predicate":"did_not_significantly_increase_expression_of","statement":"No significant GSTA1 transcriptional increase was detected in the phytochemical comparisons including DIM.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3abe9754-36f9-5853-8a3a-e53d64d49214","mechanism_event_label":"An enzyme response does not imply that every detoxification pathway rises together.","subject":{"id":"6009bdd4-f1b5-5867-b5c3-cdf7c9645cd0","slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"},"object":{"id":"35e312f9-e82c-5434-9357-1cfa61fe85e8","slug":"gsta1","display_name":"Human glutathione S-transferase A1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"3abe9754-36f9-5853-8a3a-e53d64d49214","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-hepatocyte-gsta1-null-event","event_type":"biochemical_relationship","label":"An enzyme response does not imply that every detoxification pathway rises together.","description":"No significant GSTA1 transcriptional increase was detected in the phytochemical comparisons including DIM.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6009bdd4-f1b5-5867-b5c3-cdf7c9645cd0","slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"35e312f9-e82c-5434-9357-1cfa61fe85e8","slug":"gsta1","display_name":"Human glutathione S-transferase A1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/dim-research/15672752.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"ccf97f305e0f4d5b98b172cef3a0875729e44b8f65420d87a6d725ec7207ca63\", \"start_char\": 0, \"end_char\": 1560, \"text_sha256\": \"ccf97f305e0f4d5b98b172cef3a0875729e44b8f65420d87a6d725ec7207ca63\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Quantitative gene expression and protein assessment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"DIM 10-50 micromolar","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-culture concentrations; fold mRNA induction is not the fold change in drug clearance in people.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Diindolylmethane (DIM) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Primary human hepatocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"An enzyme response does not imply that every detoxification pathway rises together.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[dim-p15672752] Phytochemical-induced changes in gene expression of carcinogen-metabolizing enzymes in cultured human primary hepatocytes. (2004). https://pubmed.ncbi.nlm.nih.gov/15672752/ DOI: 10.1080/00498250412331285481","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"CYP and phase-II enzyme expression","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6573dfd9-28d9-5206-b60f-4310881a5492","evidence_kind":"source_excerpt","locator":"Lines 415-426","start_line":415,"end_line":426,"excerpt":"### dim-hepatocyte-gsta1-null\nNo significant GSTA1 transcriptional increase was detected in the phytochemical comparisons including DIM.\nCondition category: normal\nnutrient_topic: Diindolylmethane (DIM) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: An enzyme response does not imply that every detoxification pathway rises together.\norganism: Primary human hepatocytes\ntissue_or_cell_type: CYP and phase-II enzyme expression\nexperimental_model: Quantitative gene expression and protein assessment\nlimitations: Cell-culture concentrations; fold mRNA induction is not the fold change in drug clearance in people.\nexposure: DIM 10-50 micromolar\nevidence_span: {\"source_cache\": \"artifacts/dim-research/15672752.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"ccf97f305e0f4d5b98b172cef3a0875729e44b8f65420d87a6d725ec7207ca63\", \"start_char\": 0, \"end_char\": 1560, \"text_sha256\": \"ccf97f305e0f4d5b98b172cef3a0875729e44b8f65420d87a6d725ec7207ca63\"}\n[dim-p15672752] Phytochemical-induced changes in gene expression of carcinogen-metabolizing enzymes in cultured human primary hepatocytes. 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