{"id":"b41605de-1ff6-5671-91b9-61c72b96f8fd","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-copper-dna-chemistry","predicate":"promotes","statement":"A prepared 1:2 copper(II):fisetin complex cleaved and degraded DNA in chemical assays.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"0b3fd252-2414-5077-8ecc-3a425b7a4558","mechanism_event_label":"Metal binding does not guarantee antioxidant protection.","subject":{"id":"ae5f5cec-c1ae-546a-a92e-1d41ca8856e9","slug":"fisetin-copper-ii-complexes","display_name":"Fisetin-copper(II) complexes, stoichiometry specified","entity_type_key":"small_molecule"},"object":{"id":"f5825915-c344-5020-923a-64c272f5b656","slug":"fisetin-copper-dna-cleavage","display_name":"DNA cleavage by prepared fisetin-copper complexes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0b3fd252-2414-5077-8ecc-3a425b7a4558","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-copper-dna-chemistry-event","event_type":"observed_relationship","label":"Metal binding does not guarantee antioxidant protection.","description":"A prepared 1:2 copper(II):fisetin complex cleaved and degraded DNA in chemical assays.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ae5f5cec-c1ae-546a-a92e-1d41ca8856e9","slug":"fisetin-copper-ii-complexes","display_name":"Fisetin-copper(II) complexes, stoichiometry specified","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f5825915-c344-5020-923a-64c272f5b656","slug":"fisetin-copper-dna-cleavage","display_name":"DNA cleavage by prepared fisetin-copper complexes","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2a870bb5-05a0-5e51-a70c-a9a843a8b571","slug":"copper-ii","display_name":"Copper(II) ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"DNA electrophoresis and spectroscopic assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Prepared complex exposure is not equivalent to eating copper-containing food with fisetin.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Metal binding does not guarantee antioxidant protection.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Physicochemical, antioxidant, DNA cleaving properties and antimicrobial activity of fisetin-copper chelates. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29247867/ · DOI 10.1016/j.jinorgbio.2017.12.006","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"be0eed53-b555-53a9-9c39-f0dd1cfaf794","evidence_kind":"source_excerpt","locator":"Lines 144-150","start_line":144,"end_line":150,"excerpt":"## fisetin-copper-dna-chemistry\nMetal binding does not guarantee antioxidant protection.\nA prepared 1:2 copper(II):fisetin complex cleaved and degraded DNA in chemical assays.\nModel: DNA electrophoresis and spectroscopic assays.\nLimitations: Prepared complex exposure is not equivalent to eating copper-containing food with fisetin.\nEvidence access: Primary abstract\nPhysicochemical, antioxidant, DNA cleaving properties and antimicrobial activity of fisetin-copper chelates. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29247867/ · DOI 10.1016/j.jinorgbio.2017.12.006","model_system":"DNA electrophoresis and spectroscopic assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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