{"id":"b4020d1f-c3b4-55ae-abfc-53b998356490","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-brain-exposure","predicate":"increases_tested","statement":"Oral D-aspartate at 20 mM in drinking water for one month and a separate 500 mg/kg intraperitoneal exposure increased extracellular prefrontal D-aspartate in mice.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f69a3920-ceab-5dcf-b34d-21662aac232c","mechanism_event_label":"Both tested routes changed the brain extracellular pool in mice.","subject":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"object":{"id":"1611a808-0ccd-537c-b4e6-7f7e6fea3456","slug":"mouse-pfc-extracellular-d-aspartate","display_name":"Extracellular D-aspartate in mouse prefrontal cortex","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f69a3920-ceab-5dcf-b34d-21662aac232c","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-brain-exposure-event","event_type":"observed_relationship","label":"Both tested routes changed the brain extracellular pool in mice.","description":"Oral D-aspartate at 20 mM in drinking water for one month and a separate 500 mg/kg intraperitoneal exposure increased extracellular prefrontal D-aspartate in mice.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1611a808-0ccd-537c-b4e6-7f7e6fea3456","slug":"mouse-pfc-extracellular-d-aspartate","display_name":"Extracellular D-aspartate in mouse prefrontal cortex","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse microdialysis; chronic oral and separate acute injection conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Water concentration is not a fixed mg/kg dose; human brain exposure remains unmeasured here.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"D-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Both tested routes changed the brain extracellular pool in mice.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Olanzapine, but not clozapine, increases glutamate release in the prefrontal cortex of freely moving mice by inhibiting D-aspartate oxidase activity. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28393897/ · DOI 10.1038/srep46288","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"56ec5cc2-4c8e-554e-8f79-b4181de108cc","evidence_kind":"source_excerpt","locator":"Lines 216-222","start_line":216,"end_line":222,"excerpt":"## d-aspartate-brain-exposure\nBoth tested routes changed the brain extracellular pool in mice.\nOral D-aspartate at 20 mM in drinking water for one month and a separate 500 mg/kg intraperitoneal exposure increased extracellular prefrontal D-aspartate in mice.\nModel: Mouse microdialysis; chronic oral and separate acute injection conditions.\nLimitations: Water concentration is not a fixed mg/kg dose; human brain exposure remains unmeasured here.\nEvidence access: Primary full text\nOlanzapine, but not clozapine, increases glutamate release in the prefrontal cortex of freely moving mice by inhibiting D-aspartate oxidase activity. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28393897/ · DOI 10.1038/srep46288","model_system":"Mouse microdialysis; chronic oral and separate acute injection conditions.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3751103e-e256-5615-b24f-39cc76ccfb47","stable_key":"import-9a47f338-d127-5e4d-abf6-e99056833a69","title":"D-Aspartate: synthesis, clearance, neural and endocrine mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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