{"id":"b3dd57ea-e2eb-53ec-8b8e-677d12e2528e","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-tlr4-adduct","predicate":"modifies_cysteines_on","statement":"Receptor mapping found sulforaphane adducts at TLR4 C246 and C609 under non-reducing conditions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f71316f7-85ef-5689-86a9-f5cb9ba957d6","mechanism_event_label":"The compound can interact with an inflammatory sensor as well as KEAP1.","subject":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"object":{"id":"3f792f3c-3129-5995-8341-2295c5ea5b27","slug":"tlr4","display_name":"Human toll-like receptor 4 / TLR4","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"f71316f7-85ef-5689-86a9-f5cb9ba957d6","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-tlr4-adduct-event","event_type":"biochemical_relationship","label":"The compound can interact with an inflammatory sensor as well as KEAP1.","description":"Receptor mapping found sulforaphane adducts at TLR4 C246 and C609 under non-reducing conditions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3f792f3c-3129-5995-8341-2295c5ea5b27","slug":"tlr4","display_name":"Human toll-like receptor 4 / TLR4","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/sulforaphane-research/25284333.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0a293f12863f5cfc2d57d74f4acee8c62700e2744a5900643bc23eca85c62be5\", \"start_char\": 0, \"end_char\": 1494, \"text_sha256\": \"0a293f12863f5cfc2d57d74f4acee8c62700e2744a5900643bc23eca85c62be5\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant receptor mapping, human cell exposure and ex vivo challenge","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Sulforaphane under non-reducing conditions; LPS challenge after ITC exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Covalent mapping does not establish the sole cause of cytokine effects; not a chronic-disease outcome trial.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human TLR4, THP-1 monocytes and donor PBMCs","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The compound can interact with an inflammatory sensor as well as KEAP1.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sulforaphane-p25284333] Suppression of LPS-induced transcription and cytokine secretion by the dietary isothiocyanate sulforaphane. 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