{"id":"b3b0a3b4-50ad-50ea-8a31-023386baf1ed","stable_key":"649e861b-265a-5912-bc36-3a73e53ef892:l-serine-als-serine-addition","predicate":"increases","statement":"Adding excess serine further increased already elevated sphingolipid levels in mutant-SPTLC1 human iPSC-derived motor-neuron-like cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f1f662e4-6c08-5fdc-a7c0-7e9f1c3eeba2","mechanism_event_label":"Extra substrate can feed an overactive pathway.","subject":{"id":"c882cd0b-9d83-5b07-bd1c-fe4d45657dcb","slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"},"object":{"id":"fcfaee7e-d5ec-5d69-bf7c-c7bfe04d2a13","slug":"human-sptlc1-als-motor-neuron-lipids","display_name":"Sphingolipid excess in SPTLC1-ALS motor-neuron models","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f1f662e4-6c08-5fdc-a7c0-7e9f1c3eeba2","stable_key":"649e861b-265a-5912-bc36-3a73e53ef892:l-serine-als-serine-addition-event","event_type":"observed_relationship","label":"Extra substrate can feed an overactive pathway.","description":"Adding excess serine further increased already elevated sphingolipid levels in mutant-SPTLC1 human iPSC-derived motor-neuron-like cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c882cd0b-9d83-5b07-bd1c-fe4d45657dcb","slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fcfaee7e-d5ec-5d69-bf7c-c7bfe04d2a13","slug":"human-sptlc1-als-motor-neuron-lipids","display_name":"Sphingolipid excess in SPTLC1-ALS motor-neuron models","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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