{"id":"b3a0e459-6f73-5c26-a350-e60eff61cbee","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-glucose-switch","predicate":"delays","statement":"Adding glucose prevented lactate utilization by the tested lactate-to-butyrate isolates until glucose was exhausted.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2e0ce6ca-d7f2-5107-9633-eeee55ca77f0","mechanism_event_label":"The available carbohydrate changed which substrate microbes used first.","subject":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"object":{"id":"1907485f-e027-5d85-acab-cf299fbfd169","slug":"microbial-butyrate-production","display_name":"Gut microbial butyrate production","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2e0ce6ca-d7f2-5107-9633-eeee55ca77f0","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-glucose-switch-event","event_type":"observed_relationship","label":"The available carbohydrate changed which substrate microbes used first.","description":"Adding glucose prevented lactate utilization by the tested lactate-to-butyrate isolates until glucose was exhausted.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1907485f-e027-5d85-acab-cf299fbfd169","slug":"microbial-butyrate-production","display_name":"Gut microbial butyrate production","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5ebd9a7c-b151-5b70-b70d-7d33067d4ebf","slug":"lactate","display_name":"L-Lactate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Anaerobic batch cultures.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A substrate-preference experiment does not mean dietary glucose universally suppresses human butyrate production.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Butyrate collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The available carbohydrate changed which substrate microbes used first.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Lactate-utilizing bacteria, isolated from human feces, that produce butyrate as a major fermentation product. · 2004 · https://pubmed.ncbi.nlm.nih.gov/15466518/ · DOI 10.1128/AEM.70.10.5810-5817.2004","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"071136a0-6bd9-5662-9065-d2df21f98106","evidence_kind":"source_excerpt","locator":"Lines 70-76","start_line":70,"end_line":76,"excerpt":"## butyrate-glucose-switch\nThe available carbohydrate changed which substrate microbes used first.\nAdding glucose prevented lactate utilization by the tested lactate-to-butyrate isolates until glucose was exhausted.\nModel: Anaerobic batch cultures.\nLimitations: A substrate-preference experiment does not mean dietary glucose universally suppresses human butyrate production.\nEvidence access: Primary abstract\nLactate-utilizing bacteria, isolated from human feces, that produce butyrate as a major fermentation product. · 2004 · https://pubmed.ncbi.nlm.nih.gov/15466518/ · DOI 10.1128/AEM.70.10.5810-5817.2004","model_system":"Anaerobic batch cultures.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"33205bb8-dcbe-5d2c-afca-86075d7748d1","stable_key":"import-104835a3-b891-5dc4-a6bb-42ab7c6f96c4","title":"Butyrate: microbial production, fuel use, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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