{"id":"b30f2e3e-cc96-5798-ab77-f0baf50bc6ae","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-melatonin-rescue","predicate":"attenuates_metabolite_impairment","statement":"Melatonin pretreatment reduced sulfanilic-acid-associated impairment of trypsin secretion.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"06e576d5-966b-5ec0-b7bb-5f3298e3199f","mechanism_event_label":"Melatonin pretreatment reduced sulfanilic-acid-associated impairment of trypsin secretion.","subject":{"id":"0f0bc1f1-d18d-5652-bc1d-98f61f7ab65c","slug":"melatonin","display_name":"Melatonin","entity_type_key":"small_molecule"},"object":{"id":"bd4ced0d-97de-5388-bfe3-e64b788a2f84","slug":"rat-ar42j-cck-stimulated-trypsin-secretion","display_name":"CCK-8-stimulated trypsin secretion by rat AR42J cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"06e576d5-966b-5ec0-b7bb-5f3298e3199f","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-melatonin-rescue-event","event_type":"observed_relationship","label":"Melatonin pretreatment reduced sulfanilic-acid-associated impairment of trypsin secretion.","description":"Melatonin pretreatment reduced sulfanilic-acid-associated impairment of trypsin secretion.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0f0bc1f1-d18d-5652-bc1d-98f61f7ab65c","slug":"melatonin","display_name":"Melatonin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bd4ced0d-97de-5388-bfe3-e64b788a2f84","slug":"rat-ar42j-cck-stimulated-trypsin-secretion","display_name":"CCK-8-stimulated trypsin secretion by rat AR42J cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f0f532e0-d3c7-5f4f-a9de-a979cd031bae","slug":"sulfanilic-acid","display_name":"Sulfanilic acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"3ced4333-305c-5dad-b64f-2b3585323bea","slug":"cck-8","display_name":"Cholecystokinin octapeptide / CCK-8","entity_type_key":"peptide"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Sulfanilic acid exposure; melatonin pretreatment 100 micromolar; exact CCK-8 series not in abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Pretreatment interval not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract; unrecovered method details explicitly retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific experimental curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat AR42J pancreatic cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pharmacological antioxidant rescue does not establish a clinical melatonin regimen or a specific melatonin receptor mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tartrazine food-colorant chapter; nutrient, drug and peptide interactions retain their models and limits.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rat AR42J pancreatic cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Melatonin pretreatment reduced sulfanilic-acid-associated impairment of trypsin secretion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Sulfanilic acid increases intracellular free-calcium concentration, induces reactive oxygen species production and impairs trypsin secretion in pancreatic AR42J cells. (2018). https://pubmed.ncbi.nlm.nih.gov/29986830/ DOI: 10.1016/j.fct.2018.07.001","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro pretreatment and secretagogue challenge","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"CCK-8-stimulated secretion","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4caefcaa-b7ca-5a7e-9930-e47d06a69e2e","evidence_kind":"source_excerpt","locator":"Lines 204-213","start_line":204,"end_line":213,"excerpt":"## tartrazine-melatonin-rescue\nMelatonin pretreatment reduced sulfanilic-acid-associated impairment of trypsin secretion.\nModel/species: Rat AR42J pancreatic cells\nTissue: CCK-8-stimulated secretion\nExposure: Sulfanilic acid exposure; melatonin pretreatment 100 micromolar; exact CCK-8 series not in abstract\nRoute: In vitro pretreatment and secretagogue challenge\nDuration: Pretreatment interval not specified in accessed abstract\nLimits: Pharmacological antioxidant rescue does not establish a clinical melatonin regimen or a specific melatonin receptor mechanism.\nPrimary reference: Sulfanilic acid increases intracellular free-calcium concentration, induces reactive oxygen species production and impairs trypsin secretion in pancreatic AR42J cells. (2018). https://pubmed.ncbi.nlm.nih.gov/29986830/ DOI: 10.1016/j.fct.2018.07.001\nAccess: Primary PubMed abstract; unrecovered method details explicitly retained.","model_system":"Rat AR42J pancreatic cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; primary citation retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d9540941-68b4-5a0d-a5c1-828665a918e2","stable_key":"import-792249b7-a38d-5f43-afb6-2936818a3fa2","title":"Tartrazine: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eighteen primary studies. Study-specific citations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"b83a0113a4b743adc91c24504e488f47e10665386b299c4cb0543da8948f4246","revision_id":"3769070e-6af4-5b02-9d8e-f23c84edba1b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}