{"id":"b253af6e-bc68-5867-8756-703b1c0deca7","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-aars-complementation","predicate":"rescues","statement":"A human alanyl-tRNA synthetase cDNA restored growth in yeast carrying a disrupted essential ALA1 gene, supporting conserved alanine-tRNA recognition and aminoacylation function.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0578ca02-21d1-51da-bfbe-c8ac1a2a8df5","mechanism_event_label":"Alanine must be loaded onto the correct transfer RNA before proteins can be built.","subject":{"id":"cc0bb2ab-8cf9-54a7-af05-3681ef835010","slug":"aars1","display_name":"Human cytosolic alanyl-tRNA synthetase / AARS1","entity_type_key":"protein"},"object":{"id":"5224405f-06a8-5e63-bf6b-f39bca030fa0","slug":"yeast-ala1-disruption-growth","display_name":"Growth failure after yeast ALA1 disruption","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0578ca02-21d1-51da-bfbe-c8ac1a2a8df5","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-aars-complementation-event","event_type":"observed_relationship","label":"Alanine must be loaded onto the correct transfer RNA before proteins can be built.","description":"A human alanyl-tRNA synthetase cDNA restored growth in yeast carrying a disrupted essential ALA1 gene, supporting conserved alanine-tRNA recognition and aminoacylation function.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"cc0bb2ab-8cf9-54a7-af05-3681ef835010","slug":"aars1","display_name":"Human cytosolic alanyl-tRNA synthetase / AARS1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5224405f-06a8-5e63-bf6b-f39bca030fa0","slug":"yeast-ala1-disruption-growth","display_name":"Growth failure after yeast ALA1 disruption","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a28c2f20-14aa-5b6e-ab7f-e3f7b508df66","slug":"trna-ala","display_name":"Alanine transfer RNA","entity_type_key":"rna"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human enzyme expressed in Saccharomyces cerevisiae; cross-species complementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Rescue in yeast is not a human supplementation trial. Conserved G3.U70 recognition was the study interpretation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Alanine must be loaded onto the correct transfer RNA before proteins can be built.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Wide cross-species aminoacyl-tRNA synthetase replacement in vivo: yeast cytoplasmic alanine enzyme replaced by human polymyositis serum antigen. · 1995 · https://pubmed.ncbi.nlm.nih.gov/7761427/ · DOI 10.1073/pnas.92.11.4932","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"cd4e3f24-cbf0-54cb-8521-875a575e29c6","evidence_kind":"source_excerpt","locator":"Lines 56-62","start_line":56,"end_line":62,"excerpt":"## alanine-aars-complementation\nAlanine must be loaded onto the correct transfer RNA before proteins can be built.\nA human alanyl-tRNA synthetase cDNA restored growth in yeast carrying a disrupted essential ALA1 gene, supporting conserved alanine-tRNA recognition and aminoacylation function.\nModel: Human enzyme expressed in Saccharomyces cerevisiae; cross-species complementation.\nLimitations: Rescue in yeast is not a human supplementation trial. Conserved G3.U70 recognition was the study interpretation.\nEvidence access: Primary abstract\nWide cross-species aminoacyl-tRNA synthetase replacement in vivo: yeast cytoplasmic alanine enzyme replaced by human polymyositis serum antigen. · 1995 · https://pubmed.ncbi.nlm.nih.gov/7761427/ · DOI 10.1073/pnas.92.11.4932","model_system":"Human enzyme expressed in Saccharomyces cerevisiae; cross-species complementation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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