{"id":"b2240bbb-2a31-5694-a20b-058a323a634b","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-deficiency-redcell-gsr","predicate":"marginal_deficiency_associated_with_lower_gsr","statement":"Marginally B2-deficient subjects had lower erythrocyte glutathione reductase activity than controls across the study comparison.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"06aebe44-91a2-58c6-9345-fa18d950b2a7","mechanism_event_label":"A B2-dependent recycling enzyme was less active in red cells from the deficient group.","subject":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"object":{"id":"d41f303b-1081-52f9-83a5-b14c72e9b91f","slug":"erythrocyte-gsr-activity","display_name":"Erythrocyte glutathione reductase activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"06aebe44-91a2-58c6-9345-fa18d950b2a7","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-deficiency-redcell-gsr-event","event_type":"observed_intervention","label":"A B2-dependent recycling enzyme was less active in red cells from the deficient group.","description":"Marginally B2-deficient subjects had lower erythrocyte glutathione reductase activity than controls across the study comparison.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ae04c12f-c0ac-5c8d-9ee2-76e58156d105","slug":"gsr","display_name":"Glutathione reductase / GSR","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e2cd7179-f218-54e8-9ce9-7a836ae35fac","slug":"fad","display_name":"FAD","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"measured_redox_metabolite","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"d41f303b-1081-52f9-83a5-b14c72e9b91f","slug":"erythrocyte-gsr-activity","display_name":"Erythrocyte glutathione reductase activity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"B2 supports a component of glutathione recycling; this is not proof of universal glutathione exhaustion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Erythrocytes from marginally riboflavin-deficient people and controls separated into nine density fractions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Red-cell age-fraction enzyme/cofactor assays, glutathione, hemoglobin species and peroxide susceptibility; observational comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Observational age-fraction study; reduced glutathione itself was not among the significant between-group differences.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A B2-dependent recycling enzyme was less active in red cells from the deficient group.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b2-powers1981] Riboflavin deficiency in man: effects on haemoglobin and reduced glutathione in erythrocytes of different ages (1981). https://pubmed.ncbi.nlm.nih.gov/7284295/ DOI: 10.1079/bjn19810031","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Human clinical setting","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; 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observational comparison.\ncross_nutrient: B2 supports a component of glutathione recycling; this is not proof of universal glutathione exhaustion.\n[b2-powers1981] Riboflavin deficiency in man: effects on haemoglobin and reduced glutathione in erythrocytes of different ages (1981). https://pubmed.ncbi.nlm.nih.gov/7284295/ DOI: 10.1079/bjn19810031","model_system":"Erythrocytes from marginally riboflavin-deficient people and controls separated into nine density fractions.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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