{"id":"b208d05e-00e7-5059-a069-d261f383bf4d","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-svct2-fibroblast-loss","predicate":"deletion-reduces","statement":"Embryonic fibroblasts from SVCT2-null mice retained less than 5% of normal ascorbic-acid uptake.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"19cd9b63-563e-5336-b25a-9b57ccb39ad4","mechanism_event_label":"Without SVCT2, these embryonic cells took up very little reduced vitamin C.","subject":{"id":"cdc19ebb-7348-5bd5-b0b1-87a95ecf822c","slug":"mouse-slc23a2-gene","display_name":"Mouse Slc23a2 gene","entity_type_key":"gene"},"object":{"id":"61186857-2faa-5498-9207-b637a63e4276","slug":"ascorbate-cellular-uptake","display_name":"Cellular ascorbate uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"19cd9b63-563e-5336-b25a-9b57ccb39ad4","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-svct2-fibroblast-loss-event","event_type":"biochemical_relationship","label":"Without SVCT2, these embryonic cells took up very little reduced vitamin C.","description":"Embryonic fibroblasts from SVCT2-null mice retained less than 5% of normal ascorbic-acid uptake.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cdc19ebb-7348-5bd5-b0b1-87a95ecf822c","slug":"mouse-slc23a2-gene","display_name":"Mouse Slc23a2 gene","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"61186857-2faa-5498-9207-b637a63e4276","slug":"ascorbate-cellular-uptake","display_name":"Cellular ascorbate uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"SVCT2-null mouse embryos, newborns and embryonic fibroblasts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Homozygous SVCT2 gene deletion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Historical gene naming corrected to Slc23a2; abstract-limited assay details.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin C research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-c","display_name":"Vitamin C","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Without SVCT2, these embryonic cells took up very little reduced vitamin C.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sotiriou2002] Ascorbic-acid transporter Slc23a1 is essential for vitamin C transport into the brain and for perinatal survival. (2002). https://pubmed.ncbi.nlm.nih.gov/11984597/ DOI: 10.1038/0502-514","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Embryonic fibroblasts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"c87407f3-8aea-59ac-9903-657cf69a9707","evidence_kind":"source_excerpt","locator":"Lines 208-219","start_line":208,"end_line":219,"excerpt":"### vc-transport-svct2-fibroblast-loss\nEmbryonic fibroblasts from SVCT2-null mice retained less than 5% of normal ascorbic-acid uptake.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Without SVCT2, these embryonic cells took up very little reduced vitamin C.\norganism: Mus musculus\ntissue_or_cell_type: Embryonic fibroblasts\nexperimental_model: SVCT2-null mouse embryos, newborns and embryonic fibroblasts\nlimitations: Historical gene naming corrected to Slc23a2; abstract-limited assay details.\nexposure: Homozygous SVCT2 gene deletion\ncross_nutrient: false\n[sotiriou2002] Ascorbic-acid transporter Slc23a1 is essential for vitamin C transport into the brain and for perinatal survival. (2002). https://pubmed.ncbi.nlm.nih.gov/11984597/ DOI: 10.1038/0502-514","model_system":"SVCT2-null mouse embryos, newborns and embryonic fibroblasts","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [sotiriou2002] Ascorbic-acid transporter Slc23a1 is essential for vitamin C transport into the brain and for perinatal survival. (2002). https://pubmed.ncbi.nlm.nih.gov/11984597/ DOI: 10.1038/0502-514","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"fa811221-13bd-5c10-adc1-eaf097c7703c","stable_key":"import-cb568d28-484a-5c2e-9fcc-2d780358e514","title":"Vitamin C: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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