{"id":"b1fa1088-2dbd-5cde-a5f5-b97dd741ac15","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-trans-a7-halide-current2025","predicate":"transports","statement":"Human SLC26A7 expression in HEK293T cells generated chloride and iodide currents that increased when bath halide concentrations rose from 50 to 149 mM.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"64825dc6-3031-50e3-9ffa-4c1eb1655aaf","mechanism_event_label":"Human SLC26A7 can carry both chloride and iodide under controlled laboratory conditions.","subject":{"id":"6ae2ac42-e051-59b1-aabb-f8263f987ea4","slug":"slc26a7","display_name":"Human SLC26A7 anion transporter","entity_type_key":"protein"},"object":{"id":"83b11ca6-52c7-5a1b-8c39-d9cdf89244e3","slug":"iodide","display_name":"Iodide ion","entity_type_key":"ion"},"evidence_count":1,"mechanism_event":{"id":"64825dc6-3031-50e3-9ffa-4c1eb1655aaf","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-trans-a7-halide-current2025-event","event_type":"biochemical_relationship","label":"Human SLC26A7 can carry both chloride and iodide under controlled laboratory conditions.","description":"Human SLC26A7 expression in HEK293T cells generated chloride and iodide currents that increased when bath halide concentrations rose from 50 to 149 mM.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"83b11ca6-52c7-5a1b-8c39-d9cdf89244e3","slug":"iodide","display_name":"Iodide ion","entity_type_key":"ion"},"role":"conducted_anion","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"conducted_anion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6ae2ac42-e051-59b1-aabb-f8263f987ea4","slug":"slc26a7","display_name":"Human SLC26A7 anion transporter","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human SLC26A7 cryo-EM; recombinant human SLC26A7 in HEK293T cells with whole-cell patch clamp","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Whole-cell patch clamp 24 hours after 2 micrograms plasmid transfection; 50/149 mM total halide conditions; voltage steps from −100 to +100 mV.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Halide concentrations greatly exceed physiological iodide; transport capacity does not settle net thyroid iodide flux, stoichiometry or localization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iodine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iodine","display_name":"Iodine","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Human SLC26A7 can carry both chloride and iodide under controlled laboratory conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[iodine-trans-slc26a7-structure2025] Structural basis for substrate recognition mechanism of human SLC26A7. (2025). https://pubmed.ncbi.nlm.nih.gov/40817112/ DOI: 10.1038/s41467-025-62792-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HEK293T plasma membrane","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"depends","comparator":null,"unit":null,"notes":"Recorded as a halide current that rose with bath halide concentration, so the direction follows the gradient.","entity":null},{"dimension":"transport_pool","value_text":"the cytosol across the plasma membrane","comparator":null,"unit":null,"notes":"Recorded as a halide current that rose with bath halide concentration, so the direction follows the gradient.","entity":null}],"evidence":[{"id":"8df1dff5-d6ba-56d0-82ef-92a7299806ea","evidence_kind":"source_excerpt","locator":"Lines 466-477","start_line":466,"end_line":477,"excerpt":"### iodine-trans-a7-halide-current2025\nHuman SLC26A7 expression in HEK293T cells generated chloride and iodide currents that increased when bath halide concentrations rose from 50 to 149 mM.\nCondition category: normal\nnutrient_topic: Iodine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Human SLC26A7 can carry both chloride and iodide under controlled laboratory conditions.\norganism: Homo sapiens\ntissue_or_cell_type: HEK293T plasma membrane\nexperimental_model: Purified human SLC26A7 cryo-EM; recombinant human SLC26A7 in HEK293T cells with whole-cell patch clamp\nlimitations: Halide concentrations greatly exceed physiological iodide; transport capacity does not settle net thyroid iodide flux, stoichiometry or localization.\nexposure: Whole-cell patch clamp 24 hours after 2 micrograms plasmid transfection; 50/149 mM total halide conditions; voltage steps from −100 to +100 mV.\ncross_nutrient: true\n[iodine-trans-slc26a7-structure2025] Structural basis for substrate recognition mechanism of human SLC26A7. 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